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Injectable myocardial repair hydrogel and preparation method thereof

A technology for repairing water and heart muscle, applied in the field of bionic materials, can solve the problems of unfavorable electrical signal transmission, unfavorable myocardial cell proliferation, etc., and achieve the effect of favorable myocardial cell proliferation and good cell compatibility

Inactive Publication Date: 2021-08-10
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, single-component hydrogel is usually an electrical insulating material, which is not conducive to the transmission of electrical signals between cells, and thus is not conducive to the proliferation of cardiomyocytes.

Method used

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  • Injectable myocardial repair hydrogel and preparation method thereof
  • Injectable myocardial repair hydrogel and preparation method thereof
  • Injectable myocardial repair hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] 1. Preparation of allyl gelatin

[0037] The preparation method of allyl gelatin comprises the following steps:

[0038] Dissolve 10g of gelatin in 100ml of deionized water, stir and dissolve at 50°C to obtain a gelatin solution, add 8ml of methacrylic anhydride to the gelatin solution, mix well and react at room temperature for 3 hours, then terminate the reaction with 300ml of deionized water, The solution was dialyzed for one week and freeze-dried at -80°C for use.

[0039] 2. Preparation of oxidized dextran

[0040] Dissolve 1 g of dextran in 10 ml of deionized water to obtain a dextran solution, add 2 ml of sodium periodate solution with a concentration of 100 mg / ml to the dextran solution, and react for 20 hours at room temperature in the dark, and terminate the reaction with ethylene glycol , dialyzed, and freeze-dried for later use.

Embodiment 1

[0041] Embodiment 1, an injectable myocardial repair hydrogel and its preparation method

[0042] The injectable myocardial repair hydrogel of the present invention comprises the following components in parts by weight:

[0043] 50 parts of allyl gelatin, 40 parts of oxidized dextran and 10 parts of graphene oxide.

[0044] The preparation method of the injectable myocardial repair hydrogel of the present invention comprises the following steps:

[0045] S1. Dissolve allyl gelatin in water to obtain a 50 mg / ml solution, add oxidized dextran and graphene oxide, and mix well to obtain a mixed solution A;

[0046] S2. Dissolving photoinitiator I2959 in N-methylpyrrolidone to obtain a solution with a mass concentration of 0.1%, taking 20 microliters of the solution and adding it to mixed solution A to obtain mixed solution B;

[0047] S3. Using ultraviolet light with a wavelength of 365nm to irradiate the mixed solution B for 3 minutes to obtain an injectable myocardial repair h...

Embodiment 2

[0048] Example 2, an injectable myocardial repair hydrogel and its preparation method

[0049] The injectable myocardial repair hydrogel of the present invention comprises the following components in parts by weight:

[0050] 30 parts of allyl gelatin, 40 parts of oxidized dextran and 10 parts of graphene oxide.

[0051] The preparation method of the injectable myocardial repair hydrogel of the present invention comprises the following steps:

[0052] S1. Dissolve allyl gelatin in water to obtain a 30 mg / ml solution, add oxidized dextran and graphene oxide, and mix well to obtain a mixed solution A;

[0053]S2. Dissolving photoinitiator I2959 in N-methylpyrrolidone to obtain a solution with a mass concentration of 0.1%, taking 20 microliters of the solution and adding it to mixed solution A to obtain mixed solution B;

[0054] S3. Using ultraviolet light with a wavelength of 365nm to irradiate the mixed solution B for 3 minutes to obtain an injectable myocardial repair hydro...

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Abstract

The invention relates to the technical field of biomimetic materials, in particular to injectable myocardial repair hydrogel and a preparation method thereof. The injectable myocardial repair hydrogel disclosed by the invention is prepared from the following components in parts by weight: 30 to 60 parts of allyl gelatin, 20 to 50 parts of oxidized dextran and 3 to 15 parts of graphene oxide. The injectable myocardial repair hydrogel disclosed by the invention has a conductive characteristic similar to that of myocardial cells and is beneficial to transmission of electric signals among the cells, the flexibility of the injectable myocardial repair hydrogel better conforms to natural myocardium, and the injectable myocardial repair hydrogel is beneficial to proliferation of the myocardial cells.

Description

technical field [0001] The invention relates to the technical field of bionic materials, in particular to an injectable myocardial repair hydrogel and a preparation method thereof. Background technique [0002] Native myocardium is an electrically active tissue in which Purkinje fibers with electrical conduction function are distributed. When the electrical signal from the sinoatrial node passes through the myocardium, it induces the synchronous contraction of cardiomyocytes through the "excitation-contraction" coupling effect. At the same time, myocardial tissue also has excellent mechanical strength, which can support the continuous contraction and relaxation of the heart beat. Therefore, the scaffold material used for the construction of engineered myocardial tissue should have both excellent electrical conductivity and elastic modulus. [0003] Biological scaffolds play a key role in tissue engineering reconstruction. They can be used as drug carriers and create a biom...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/08A61L27/22A61L27/20A61L27/50C08J3/075C08J3/24C08L89/00C08L5/02
CPCA61L27/52A61L27/08A61L27/222A61L27/20A61L27/50C08J3/075C08J3/246A61L2430/20A61L2400/06C08J2389/00C08J2405/02
Inventor 郭瑞冯龙宝蓝咏刘玉
Owner BEOGENE BIOTECH GUANGZHOU
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