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SNP marker related to growth rate of leiocassis longirostris and application thereof

A technology of growth speed and long-breasted catfish, applied in the field of SNP markers, can solve the problems of SNP markers that need to be excavated, and achieve the effect of fast growth and accelerated cultivation process

Pending Publication Date: 2021-08-06
SOUTHWEST UNIVERSITY +2
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Problems solved by technology

SNP markers are the most efficient markers for molecular assisted breeding after microsatellite markers. However, SNPs related to the growth (body weight, total length, body length, and head height) of S. longissimus have not been reported, and they can be effectively used for the growth of S. longissimus. SNP markers for breeding related traits need to be discovered

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  • SNP marker related to growth rate of leiocassis longirostris and application thereof
  • SNP marker related to growth rate of leiocassis longirostris and application thereof
  • SNP marker related to growth rate of leiocassis longirostris and application thereof

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Embodiment Construction

[0028] Embodiments of the technical solution of the present invention will be described in detail below. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and therefore are only examples, rather than limiting the protection scope of the present invention.

[0029] 1.1 Sources of samples from the long-snouted catfish population

[0030] The long-nosed catfish to be tested came from the rare fish protection base in the middle reaches of the Minjiang River of the Fisheries Research Institute of the Sichuan Academy of Agricultural Sciences. Randomly selected 115 long-nosed catfish from the same batch of artificial breeding and the same feeding conditions, and measured their phenotypic traits (body weight, length, body length, head height), and the dorsal fin rays were cut and stored in absolute ethanol for genomic DNA extraction.

[0031] 1.2 Extraction of Genomic DNA from the Long-snout Catfish to be tested

[0032...

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Abstract

The invention provides an SNP marker related to the growth rate of leiocassis longirostris and application of the SNP marker. The SNP marker comprises an SNP1 marker and an SNP2 marker, and nucleotide sequences of the SNP1 marker and the SNP2 marker are shown as SEQ ID NO.1 and SEQ ID NO.2 in sequence. When the genotype of the SNP1 site is mutated homozygous TT and the genotype of the SNP2 site is mutated homozygous GG, the growth speed of the individual leiocassis longirostris to be detected is higher. The individuals with the genotype of TT at the SNP1 site and the individuals with the genotype of GG at the SNP2 site are selected to be hybridized, and the offspring individuals are of a rapid growth type. The SNP marker disclosed by the invention is closely related to the growth speed of the leiocassis longirostris, parents can be selected according to breeding requirements in the early stage of fry culture, and the marker disclosed by the invention is used for assisted breeding, so that the culture process of good varieties of the leiocassis longirostris is accelerated.

Description

technical field [0001] The present invention relates to SNP markers related to the growth rate of long-breasted catfish and applications thereof, in particular to SNP markers related to the growth rate of long-breasted catfish, primer pairs and kits for detecting the aforementioned SNP markers, and the aforementioned SNP markers, primer pairs and reagents The use of the box in the selection and breeding of long-snout catfish, and the method for detecting the growth rate of long-snout catfish. Background technique [0002] Leiocassis longirostris, also known as catfish, belongs to the order Siloriformes, the family Bagridae, and the genus Leiocassis. Because its snout is longer than that of ordinary fish, it is named long-nose longirostris. The long-snouted squid has tender meat, smooth taste, less intermuscular thorns, and rich nutrition. There is a folk saying that "if you don't eat Jiangtuan, you don't know the taste of fish". delicacies. Long-snouted squid is a rare and...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122C12Q2565/627Y02A40/81
Inventor 罗辉周剑叶华李雨李哲魏震
Owner SOUTHWEST UNIVERSITY
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