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Application of streptococcus thermophilus MN002 in lipid metabolism regulation and dietary supplement

A technology of Streptococcus thermophilus and dietary supplements, applied in the field of microorganisms, can solve problems such as product instability, susceptibility to antibiotics, risk of bacteriophage infection and outbreak, and achieve excellent heat resistance and pH stability, without resistance The risk of drug gene transfer and the effect of reducing fat cell accumulation

Active Publication Date: 2021-08-06
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]The other category is probiotic products, all of which are live bacterial preparations. This type of live bacterial preparations has the following many shortcomings that affect the efficacy of the product, including: (1) When the intestinal tract is immature, inflamed or immune insufficiency, live bacteria will break through the intestinal wall and invade the body, causing inflammation; (2) susceptible to antibiotics, and easy to cause drug resistance gene transfer; (3) long-term use Live bacterial preparations increase the risk of phage infection and outbreak; (4) poor processing characteristics such as heat resistance, easy pollution, and product instability; (5) low temperature, dark and other product storage conditions are relatively harsh

Method used

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  • Application of streptococcus thermophilus MN002 in lipid metabolism regulation and dietary supplement
  • Application of streptococcus thermophilus MN002 in lipid metabolism regulation and dietary supplement
  • Application of streptococcus thermophilus MN002 in lipid metabolism regulation and dietary supplement

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preparation example Construction

[0051] The preparation method of the inactivated Streptococcus thermophilus MN002 bacterium powder in following embodiment, comprises:

[0052] (1) Activate the preserved strain Mn 002 with MRS liquid medium, culture it statically at 37°C for 16-20 hours, cultivate it to the logarithmic phase, and the number of viable bacteria reaches 10 9 More than cfu / mL;

[0053] (2) Collect the wet bacteria by centrifugation, add a protective agent, and vacuum-dry at 70-80° C. to obtain bacteria powder with inactivated Streptococcus thermophilus MN002.

Embodiment 1

[0055] Dietary supplements with lipid metabolism regulation function, including skimmed milk powder, maltodextrin, resistant dextrin, isomalto-oligosaccharide, fructo-oligosaccharide, stachyose, lactitol, citric acid, inulin, black soybean powder, melamine Live Streptococcus thermophilus MN002. The specific configuration is shown in Table 1.

[0056] Wherein, the preparation method of the inactivated Streptococcus thermophilus MN002 is as follows:

[0057] (1) Activate the preserved strain Mn 002 with MRS liquid medium, culture it statically at 37°C for 18 hours, cultivate it to the logarithmic phase, and the number of viable bacteria reaches 10 9 More than cfu / mL;

[0058] The formula of above-mentioned MRS liquid culture medium is:

[0059]

[0060]

[0061] Dilute to 1L and adjust the pH to 6.2.

[0062] (2) Collect the wet thalline by centrifugation, add a protective agent, and dry in vacuum at 75° C. to obtain a bacterial powder with inactivated Streptococcus th...

Embodiment 2

[0072] The difference in this embodiment is only that the composition and proportioning in Example 6 are adopted in this embodiment, the difference is only that MN002 in this embodiment is a live bacteria preparation, and the preparation method of the MN002 live bacteria preparation is as follows:

[0073] The activated Streptococcus thermophilus MN002 was cultured in two stages at 40°C for 8 hours; the culture was inserted into the enrichment medium with an inoculated amount of 2.5%, at a temperature of 40°C and a pH value of 7.1 , stirred and fermented for 18 hours to obtain the fermentation broth of Streptococcus thermophilus; centrifuged the fermentation broth of Streptococcus thermophilus MN002 at 4°C for 18 minutes, and removed the lower layer of precipitate for later use; after fully mixing the precipitate with the lyoprotectant, put it at -50 The Streptococcus thermophilus MN002 bacterial powder can be obtained by vacuum freeze-drying at ℃ for 12 hours, and the number o...

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Abstract

The invention discloses an application of streptococcus thermophilus MN002 in lipid metabolism regulation and a dietary supplement, and discloses an application of streptococcus thermophilus MN002 in preparation of a product with a lipid metabolism regulation function. The product is used for inhibiting expression of fat related transcription factors, inhibiting preadipocytes from differentiating into mature adipocytes, and further reducing fat cell accumulation and reducing secretion of fat-related cell factors. The invention also provides the dietary supplement which contains the inactivated streptococcus thermophilus MN002 and has a lipid metabolism regulation function. The effect of regulating lipid metabolism is achieved.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the use of strains in regulating lipid metabolism, and also to dietary supplements added with strains. Background technique [0002] Obesity is a metabolic disease caused by the imbalance between energy intake and consumption. In developed countries, obesity is one of the main reasons for the rising incidence of cardiovascular diseases, type 2 diabetes and certain tumors. More and more research results show that adipose tissue is not only a passive energy storage organ, but also an endocrine organ that can secrete a variety of hormones; Fatty liver, hyperlipidemia and breast cancer are closely related. [0003] The essence of cellular differentiation is a shift in gene expression patterns. Cellular fat accumulation and morphological changes accompanying lipogenesis are due to the induced expression of specific genes during cell differentiation. In this sense, the expression and a...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L33/21A23C9/152A23C9/156A61K35/744A61P3/04C12N1/20C12R1/46
CPCA23L33/135A23L33/21A23C9/152A23C9/156A61K35/744A61P3/04C12N1/20A23V2002/00A23V2400/249A23V2200/332A23V2250/284A23V2250/632A23V2250/6414A23V2250/5062
Inventor 李周勇陈建国康小红
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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