Pass cell line of porcine DC cells as well as establishment method and application thereof

A technology for establishing methods and subculture cells, applied in cell dissociation methods, methods based on microorganisms, animal cells, etc., can solve problems such as inability to pass on for a long time, and achieve the effect of eliminating dependence

Pending Publication Date: 2021-07-30
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, primary cells isolated from animals can only be passaged for a few times, and cannot be passed down for a long time

Method used

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  • Pass cell line of porcine DC cells as well as establishment method and application thereof
  • Pass cell line of porcine DC cells as well as establishment method and application thereof
  • Pass cell line of porcine DC cells as well as establishment method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] A method for establishing a passage cell line of pig DC cells, the specific steps are as follows:

[0082] Step S1: Cell Culture

[0083] (1) Separation of mononuclear cells from porcine peripheral blood:

[0084] ① Take fresh EDTA anticoagulated whole blood and dilute the whole blood with an equal volume of diluent or PBS;

[0085] ② Add an appropriate amount of separation liquid to the centrifuge tube. When the diluted blood volume is less than 3 mL, add 3 mL of separation liquid; , otherwise it will affect the separation effect, spread the diluted blood on top of the liquid surface of the separation liquid, and pay attention to keep the interface between the two liquid surfaces clear;

[0086] ③At room temperature, centrifuge with a horizontal rotor of 500-1000g for 20-30min. The larger the volume of blood, the greater the centrifugal force required and the longer the centrifugation time. The best centrifugation conditions depend on the actual situation. The maximu...

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Abstract

The invention belongs to the field of biological immunology, and particularly relates to a porcine DC cell passage cell line as well as an establishment method and application thereof. Mononuclear cells in porcine blood are differentiated into dendritic cells, a cell colony is found in the passage process, and the porcine dendritic cell line is established after repeated passage. By studying the growth, surface marking, phagocytosis and antigen presentation effects of passage cells, it is found that the cell line has the characteristics of dendritic cells and has good effects of stimulating T cell proliferation, secreting cell factors and stimulating CTL cytotoxicity.

Description

technical field [0001] The invention belongs to the field of biological immunology, and in particular relates to a passage cell line of porcine DC cells and its establishment method and application. Background technique [0002] Dendritic cells (DC) are important antigen-presenting cells and bridge cells connecting the body's innate and adaptive immune responses. DC cells recognize and phagocytize pathogens through surface receptors, participate in antigen presentation, mature, and secrete different types of cytokines, determine the direction of T cell differentiation, and ultimately affect the effect of cellular immune responses. [0003] DC cells are professional antigen-presenting cells with the most powerful functions in the body. They can efficiently absorb, process and present antigens. Immature DC cells have strong migration ability, and mature DC cells can effectively activate naive T cells. It is at the center of initiating, regulating, and maintaining the immune r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784C12N5/078C12R1/91
CPCC12N5/0634C12N5/0639C12N2501/22C12N2501/2304C12N2509/00
Inventor 王钦富刘欢柏显昌张正韬王美辰胡琳珑
Owner DALIAN UNIV
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