Application of Xianglian product or combination of Xianglian product and antibiotics in anti-helicobacter pylori drugs
A technology of Helicobacter pylori and its products, which is applied in antibacterial drugs, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., which can solve the problems of poor safety and achieve high safety and simple preparation process , Improve the effect of antibacterial activity
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Embodiment 1
[0044] Weigh 80 g of Yuhuanglian, extract by percolation with 0.5% sulfuric acid solution, until the color of the percolation liquid is very light (the total alkaloids in the extracted raw materials are more than 95% according to HPLC analysis); the percolation liquid is neutralized to pH=3.0 with calcium hydroxide , filtered; the filtrate was concentrated under reduced pressure to 1g Yuhuanglian / mL, cooled, and filtered; 10% sodium chloride was added to the filtrate, and stood at 5°C for 24 hours, precipitated and filtered to obtain extract Ⅰ; the filtered mother liquor was added 10% sodium chloride, and 0.5% zinc chloride, stand still at 5°C for 5 hours, precipitate and filter to obtain extract II; combine extract I and extract II precipitate to obtain Yuhuanglian extract.
[0045] Weigh 20g of Muxiang, extract with 5 times of water reflux for 1 hour, and collect volatile oil; filter the extracted aqueous solution, and then reflux the filtered residue with an appropriate amou...
Embodiment 2
[0048] Weigh 80 g of Yuhuanglian, extract by percolation with 2% sulfuric acid solution, until the color of the percolate is very light (the total alkaloids in the extracted raw materials are more than 95% according to HPLC analysis); the percolate is neutralized to pH=7.0 with calcium hydroxide , filtered; the filtrate was concentrated under reduced pressure to 2g Yuhuanglian / mL, cooled, and filtered; 1% potassium bromide was added to the filtrate, and stood still for 96 hours at 25°C, precipitated and filtered to obtain extract Ⅰ; the filtered mother liquor was added 20% potassium bromide, and 0.1% ferric bromide, stand at 25°C for 1 hour, precipitate and filter to obtain extract II; combine extract I and extract II precipitate to obtain Yuhuanglian extract.
[0049] Weigh 20g of Muxiang, extract with 5 times of water reflux for 1 hour, and collect volatile oil; filter the extracted aqueous solution, and then reflux the filtered residue with an appropriate amount of water to ...
Embodiment 3
[0052] Weigh 80 g of Yuhuanglian, extract by percolation with 1% sulfuric acid solution, until the color of the percolate is very light (the total alkaloids in the extracted raw materials are more than 95% according to HPLC analysis); the percolate is neutralized to pH=5.0 with calcium hydroxide , filtered; the filtrate was concentrated under reduced pressure to 1g Yuhuanglian / mL, cooled, and filtered; 10% sodium iodide was added to the filtrate, and stood at 10°C for 48 hours, precipitated and filtered to obtain extract Ⅰ; the filtered mother liquor was added 10% sodium iodide, and 0.5% magnesium iodide, stand still at 10°C for 10 hours, precipitate and filter to obtain extract II; combine extract I and extract II precipitate to obtain Yuhuanglian extract.
[0053] Weigh 20g of Muxiang, extract with 5 times of water reflux for 1 hour, and collect volatile oil; filter the extracted aqueous solution, and then reflux the filtered residue with an appropriate amount of water to ext...
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