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Serum lipid metabolite composition, kit and application

A technology for serum lipids and metabolites, applied in analytical chemistry, clinical medicine and medical fields, to achieve good application prospects, high sensitivity and specificity, and high specificity.

Active Publication Date: 2021-07-20
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, few studies have identified suitable biomarkers for use in clinical trials for lung cancer diagnosis

Method used

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  • Serum lipid metabolite composition, kit and application
  • Serum lipid metabolite composition, kit and application
  • Serum lipid metabolite composition, kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Collection of Serum Samples

[0025] Before sampling, all volunteers included in the study signed the informed consent. Fasting serum samples from 63 female healthy controls and 64 lung cancer patients (including non-smoking females with stage 0, stage I, stage II and stage III-IV lung cancer) were collected. All subjects fasted for more than 8 hours, and fasting blood was collected. After blood collection, the serum was separated and stored in a -80°C refrigerator.

[0026] 2. Analysis method

[0027] 2.1 Pretreatment of serum samples

[0028] Thaw the blood sample at 4°C, take 40 μL serum sample, add 300 μL methanol solution containing internal standard 6.7 μg / mL palmitic acid-16,16,16-d 3 and 3.3 μg / mL phosphatidylethanolamine PE15:0 / 15:0 , then rotate for 1 min, and then add 1 mL of methyl tert-butyl ether to fully extract the lipids. Then 300 μL of water was added for phase separation. The mixture was centrifuged at 10000g, 4°C for 10 minutes. The superna...

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Abstract

The invention discloses a novel serum lipid metabolite composition and application of the serum lipid metabolite composition as a marker in preparation of a non-smoking female lung cancer diagnostic kit. The serum lipid metabolite composition comprises eicosadienoic acid FA C20:2, eicosatetraenoic acid FA C20:4, tetracosatetraenoic acid FA C24:4, phosphatidylcholine PC 20:1 / 22:6, phosphatidylinositol PI18:0 / 20:5 and phosphatidyl ethanolamine PE18:0p / 22:4. The serum lipid metabolite composition can be used for auxiliary diagnosis of non-smoking female lung cancer, and has the characteristics of low detection cost, good repeatability, and high sensitivity and specificity. Meanwhile, the lipid metabolite composition also has a good effect on diagnosis of early-stage lung cancer of non-smoking females.

Description

technical field [0001] The invention relates to the application of a new serum combined marker in the diagnosis of lung cancer in non-smoking women. It belongs to the field of analytical chemistry, clinical medicine and medicine. Background technique [0002] Lung cancer is one of the malignant tumors with high morbidity and mortality in the world (Document 1: Yu et al., Globallipidomics reveals two plasma lipids as novel biomarkers for the detection of squamous cell lung cancer: A pilot study, oncol.lett.16 (2018) 761-768.). In recent years, the incidence of lung cancer in women has increased, even though most women do not smoke. Lung cancer is a heterogeneous disease with multiple subtypes with distinct histological and molecular biological features. In addition, female lung cancer appears to differ from males in terms of environmental risk factors and histologic type. Lung cancer among non-smokers has obvious gender bias, women are more likely to suffer from non-smoki...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57488G01N33/57423
Inventor 许国旺哈马达努尔刘心昱
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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