Pathological picture three-dimensional reconstruction method

Abstract

The invention discloses a pathological picture three-dimensional reconstruction method in the technical field of pathological picture display. The method comprises the following steps: S1, fixing a tumor specimen; S2, dehydrating the tumor specimen; S3, transparentizing the tumor specimen; S4, performing wax dipping on the tumor specimen; S5, embedding the tumor specimen; S6, carrying out linear bio-labeling on rows; S7, performing continuous slicing on rows; S8, carrying out HE dyeing and flaking; S9, performing immunohistochemical staining and flaking; S10, performing molecular biological detection and flaking; S11, observing with a microscope; and S12, synthesizing a three-dimensional picture. According to the method, on the basis of the two-dimensional pictures, continuous slicing and accurate positioning of the pathological specimen are carried out, so that after the tumor leaves the body, three-dimensional reconstruction of a plurality of fault pictures and three-dimensional reconstruction of the pathological picture are finally realized through sequential listing, proofreading and accurate positioning of the two-dimensional pictures in a pathological space; pathologists can observe more complete tumor tissue images as much as possible, and more complete and accurate pathology reports are provided for patients and clinicians.

Description

technical field [0001] The invention relates to the technical field of displaying pathological pictures, in particular to a method for three-dimensional reconstruction of pathological pictures. Background technique [0002] In the medical field, doctors often rely on pathological slices when judging, attributing, and treating tissue diseases. Pathological slices are similar to CT scans, which are obtained by taking a certain length of a tissue slice and imaging it under a microscope layer by layer according to the vertical direction. Sequence Diagram. [0003] Traditional pathological two-dimensional pictures lack intuition, and pathological diagnosis is only for observation and diagnosis of several local sections. There are missing points, and the understanding of diseases is lacking in comprehensiveness. At the same time, due to the high professionalism of pathology, the pathological pictures on the pathology report are complicated and difficult to understand. For the gen...

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Problems solved by technology

[0003] Traditional pathological two-dimensional pictures lack intuition, pathological diagnosis is only for observation and diagnosis of several local sections, there are loopholes, and the understanding of diseases is lacking in comprehensiveness

At the same time, due to the high professionalism of pathology, the pathological pictures on the pathology report are complicated and difficult to understand. For the general population and most other professional medical staff who lack pathological knowledge, they want to determine the benign and malignant tumors after observing the two-dimensional pictures. , invasiveness, and growth trends are more difficult

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