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Rhizoma fagopyri dibotryis extract as well as preparation method and application thereof

An extract, the technology of golden buckwheat, is applied in the field of whitening plant extract and its preparation, which can solve the problems of no whitening activity reports and achieve good whitening effect

Pending Publication Date: 2021-07-20
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Golden eriothroxanthin has anti-allergic and anti-inflammatory activities. It has been reported in the field of cosmetics that it can effectively protect against damage caused by ultraviolet rays, has strong ultraviolet absorption, anti-inflammatory, and anti-allergic effects, but it has not been seen. Whitening activity report

Method used

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  • Rhizoma fagopyri dibotryis extract as well as preparation method and application thereof
  • Rhizoma fagopyri dibotryis extract as well as preparation method and application thereof
  • Rhizoma fagopyri dibotryis extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Preparation of Golden Buckwheat Extract

[0016] (1) Put the golden buckwheat powder in a 60% ethanol solution and extract by percolation to obtain an ethanol extract, recycle the ethanol solvent to obtain the first extract; (2) add a small amount of water to the first extract , to form a suspension, and adjust the pH value of the suspension between 4.5 and 6.0; (3) add an appropriate amount of cellulase to the suspension, and react in a water bath at 45°C for 60 minutes; (4) transfer the reaction solution to a Soxhlet extractor, add 95 % ethanol, leached for 90min, filtered and separated after cooling, and the filtrate was concentrated to prepare the target extract (hereinafter referred to as sample 1).

Embodiment 2

[0017] Example 2 Comparison of Different Preparation Methods of Golden Buckwheat Extract

[0018] Put golden buckwheat powder in 60% ethanol solution and extract by percolation method to obtain ethanol extract, recover ethanol solvent, and obtain ethanol extraction sample (hereinafter referred to as sample 2)

[0019] The golden buckwheat powder is placed in 60% ethanol solution and extracted by percolation to obtain ethanol extract, and the ethanol solvent is recovered to obtain the first extract, the first extract is extracted with ethyl acetate, the extracts are combined, and recovered to obtain Ethyl acetate sample (hereinafter referred to as sample 3)

[0020] The golden buckwheat powder is placed in 60% ethanol solution and extracted by percolation to obtain ethanol extract, and the ethanol solvent is recovered to obtain the first extract, the first extract is extracted with n-butanol, the extracts are combined, and recovered to obtain n-butanol sample (hereinafter refe...

Embodiment 3

[0027] Embodiment 3 Different preparation methods tyrosinase activity comparison

[0028] As a person skilled in the art, everyone knows that the parts extracted by different extraction methods contain different compounds and have different activities. On the premise that all the chemical components are not clear, the activities of the samples prepared in Example 1 and Example 2 were compared through the tyrosine activity.

[0029] Determination of tyrosinase (TYR) activity: Measure 3 mL of 50 mmol / L PBS (pH=6.8), add 2.9 mL of 1 mmol / L levodopa solution, place in a 32°C water bath for 20 min, add Mix 0.1 mL of TYR solution (0.01 g / L), and measure the absorbance curve of the mixture with time under constant temperature conditions. It is stipulated that the absorbance value of the reaction solution at a wavelength of 475 nm increases by 0.001 per minute as a TYR activity unit (U / min), and kojic acid is used as a positive control.

[0030] Calculate the tyrosinase inhibition r...

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PUM

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Abstract

The invention provides a wild buckwheat rhizome extract as well as a preparation method and application thereof, and belongs to the technical field of plant extracts, the wild buckwheat rhizome extract comprises the following components: 4.19 to 10.44 mg / g of epicatechin, 2.48 to 7.79 mg / g of proanthocyanidin B4, 2.05 to 9.98 mg / g of catechin, 2.00 to 4.67 mg / g of gallic acid, 81 to 128 [mu] g / g of proanthocyanidin B and 1.97 to 10.37 [mu] g / g of chrysoeriol. The extract is prepared by a percolation-assisted enzymolysis process. The rhizoma fagopyri dibotryis extract can achieve a whitening effect by inhibiting the activity of tyrosinase.

Description

technical field [0001] The invention belongs to the technical field of plant extracts, and in particular relates to a whitening plant extract and its preparation method and application. Background technique [0002] With the improvement of people's living standards and aesthetic standards, smooth and fair skin is more and more respected by women, and the proportion of whitening products in the daily chemical product market is also increasing. Due to the safe and healthy performance of natural functional substances, daily chemical products containing natural plant whitening functional ingredients have gradually become the consumer objects that people respect and the product orientation of the industry. The commonly used commercialized natural whitening extract is glabra root extract , peony root extract and tea extract etc. [0003] Tyrosinase inhibitors from natural sources are attracting more and more attention due to their safety, effectiveness, and ability to effectively...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/49A61K8/368A61Q19/02
CPCA61K8/9789A61K8/498A61K8/368A61Q19/02A61K2800/782A61K2800/805A61K2800/5922
Inventor 韦敏汤毅宋萍萍汤承翰颜露钱怡云王晨
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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