Method for constructing electrochemical nucleic acid sensor recognition element through insertion method

Abstract

The invention belongs to the technical field of biosensor construction and particularly discloses a method for constructing an electrochemical nucleic acid sensor recognition element through an insertion method. The insertion method specifically comprises the following steps of inserting a sulfhydrylated capture probe into a diluent sulfydryl layer to construct an electrochemical nucleic acid sensor recognition element. Compared with an existing backfilling method, the electrochemical nucleic acid sensor recognition element prepared by adopting the insertion method can improve uniformity of the recognition element and maintain hybridization activity of the probe so that the electrochemical nucleic acid sensor recognition element has better analysis performance; meanwhile, thermodynamic and kinetic parameters of a hybridization reaction process between the capture probe and target nucleic acid in a solution are analyzed in combination with a timing coulometry, and the insertion method disclosed by the invention is more beneficial to understanding a mechanism of an interface hybridization reaction on a molecular level.

Description

technical field [0001] The invention relates to the technical field of biosensor construction, in particular to a method for constructing an electrochemical nucleic acid sensor recognition element by an insertion method. Background technique [0002] Nucleic acid biosensor is a new sensor technology gradually developed in the mid-1990s. Nucleic acid biosensors use DNA as a sensitive element, and convert the biological signal of the interaction between DNA and the substance to be measured into a measurable signal through a transducer. Compared with traditional methods, DNA biosensors have the characteristics of rapidity, sensitivity, simple operation, strong specificity, and suitable for POCT detection. In recent years, the application of DNA biosensors in the fields of gene diagnosis, environmental monitoring, and drug research has received extensive attention. According to different transducers, DNA biosensors can be classified into electrochemical, fluorescent, quartz cr...

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Problems solved by technology

However, the homogeneity of the sensor recognition element prepared by the backfill method is not enough, and, due to charge repulsion and interface effects, the interaction between the probe and the target nucleic acid is hindered, so that the hybridization efficiency between the nucleic acid on the surface of the electrochemical nucleic acid sensor is significantly low Hybridization efficiency in solution, thus affecting the analytical performance of electrochemical sensors

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