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Procoagulant Chinese redbud polysaccharide as well as extraction and separation method and application thereof

A separation method, the technology of Bauhinia, is applied in the direction of blood diseases, extracellular fluid diseases, drug combination, etc., to achieve a good effect of promoting blood coagulation

Active Publication Date: 2021-07-16
郑州奥卡优医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Regarding the polysaccharide from Bauhinia flower, there is no research on the structure and activity of the polysaccharide isolated from Bauhinia flower

Method used

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  • Procoagulant Chinese redbud polysaccharide as well as extraction and separation method and application thereof
  • Procoagulant Chinese redbud polysaccharide as well as extraction and separation method and application thereof
  • Procoagulant Chinese redbud polysaccharide as well as extraction and separation method and application thereof

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Embodiment Construction

[0023] The technical content of the present invention will be further described in detail below through specific embodiments, but the protection scope of the present invention is not limited thereto.

[0024] In the following examples, the material used is Bauhinia japonica collected in the Botanical Garden of Henan University, Kaifeng City, Henan Province. Cercis Chinensis Bunge flowers.

[0025] The ethanol concentration in this article is the volume concentration.

[0026] 1. A method for extracting and separating polysaccharides from the Bauhinia flower that promotes blood coagulation, comprising the following steps:

[0027] (1) Fresh Bauhinia flowers were dried in the shade, soaked in petroleum ether at room temperature to degrease 3 times, 24 hours each time, and the residue was evaporated to dry petroleum ether, then leached with 75% ethanol at room temperature twice, 2 days each time, to recover Bauhinia The flower residue was placed in a fume hood until it evapora...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to procoagulant Chinese redbud polysaccharide as well as an extraction and separation method and application thereof. The extraction and separation method comprises the following steps: degreasing Chinese redbud, performing water extraction and alcohol precipitation to obtain crude polysaccharide, and further purifying the deproteinized crude polysaccharide by using a DEAE-52 cellulose chromatographic column and a Sephadex G-100 gel chromatographic column. The in-vitro blood coagulation effect of the polysaccharide is investigated, and the result shows that the polysaccharide has a good blood coagulation promoting effect and can be used for preparing blood coagulation promoting drugs.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a coagulation-promoting redbud flower polysaccharide, an extraction and separation method and application thereof. Background technique [0002] Bauhinia ( Cercis Chinensis Bunge) belongs to the genus Bauhinia of the leguminous family, and is widely cultivated in southeastern my country. This species is a beautiful woody flower plant. The bark can be used as medicine. It has the effects of clearing heat and detoxifying, promoting blood circulation and promoting qi, reducing swelling and relieving pain. Phytochemical studies have shown that Bauhinia contains flavonoids, polyphenols, acids, steroids, lignans, volatile oils and pigments, and has antioxidant, tyrosinase, α - Glucosidase inhibitory activity, etc. Regarding the polysaccharide from Bauhinia flower, there is no research on the structure and activity of the polysaccharide isolated from Bauhinia f...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P7/04
CPCC08B37/0003A61P7/04
Inventor 尹震花康文艺张伟张娟娟陈林郭庆丰孙凯
Owner 郑州奥卡优医药科技有限公司
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