Procoagulant ligustrum lucidum ait flower polysaccharide as well as extraction and separation method and application thereof

Abstract

The invention belongs to the technical field of phytoextraction and specifically relates to procoagulant ligustrum lucidum ait flower polysaccharide as well as an extraction and separation method andapplication thereof. The extraction and separation method comprises the following steps: degreasing ligustrum lucidum ait flowers and performing water extraction and alcohol precipitation, so as to obtain crude polysaccharide; further purifying the deproteinized and decolored crude polysaccharide with a DEAE-52 cellulose chromatographic column and a Sephadex G-100 gel chromatographic column, so asto obtain the procoagulant ligustrum lucidum ait flower polysaccharide. According to the extraction and separation method, the in-vitro blood coagulation effect of the polysaccharide is studied; results indicate that the polysaccharide has a good procoagulant effect and can be used for preparing a procoagulant drug.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a blood coagulation-promoting privet flower polysaccharide and its extraction and separation method and application. Background technique [0002] Privet ( Ligustrum lucidum Ait) is a plant of the genus Ligustrum in the family Oleaceae. It is produced in the south of the Yangtze River to South China and Southwest provinces, and it is distributed to Shaanxi and Gansu in the northwest. It is also distributed in Korea and cultivated in India and Nepal. Its dried fruit is Ligustrum lucidum, which has the effects of nourishing liver and kidney, and black hair. At present, some researchers at home and abroad have conducted in-depth research on the processing methods, pharmacological effects, and chemical components of Ligustrum lucidum. Chemical studies have shown that it contains active ingredients such as polysaccharides, organic acids, terpenoids, steroids, an...

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Problems solved by technology

At present, some researchers at home and abroad have conducted in-depth research on the processing methods, pharmacological effects, and chemical components of Ligustrum lucidum. Chemical studies have shown that it contains active ingredients such as polysaccharides, organic acids, terpenoids, steroids, and flavonoids. , has the effects of anti-inflammatory, hypoglycemic, improving immunity and liver protection, while the research on privet flower is limited to extraction process, volatile oil, flavonoids, terpenoids, etc., and there is no research on privet flower polysaccharide reported, so this is not only an advancement in the field of polysaccharides, but also provides a broad prospect for the further development and utilization of privet

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