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Composition of Tenacissoside H and cis-platinum and application thereof

A technology for clearing vine glycosides and compositions, which is applied in the field of vine glycoside H and cisplatin compositions to achieve the effects of increasing apoptosis rate, strengthening effect, and improving quality of life

Inactive Publication Date: 2021-07-16
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report about the composition of glucoside H and cisplatin of the present invention and its application

Method used

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  • Composition of Tenacissoside H and cis-platinum and application thereof
  • Composition of Tenacissoside H and cis-platinum and application thereof
  • Composition of Tenacissoside H and cis-platinum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 Effect embodiment

[0037] 1 Experimental materials

[0038] Tongguan vine glycoside H was purchased from Baoji Chenguang Biotechnology Co., Ltd.; dimethyl sulfoxide (DMSO) was purchased from Sigma; cisplatin (CDDP) was purchased from MCE; DMEM high-glucose medium, fetal bovine serum, double antibody , PBS were purchased from Gibco; BCA kits were purchased from Solebol; CCK-8 kits were purchased from Dojindo; Annexin V-FITC / PI apoptosis kits were purchased from BD, USA.

[0039] 2 Experimental methods

[0040] 2.1 Cell culture

[0041]Take out the cryopreservation tube containing the cells from the liquid nitrogen tank, quickly put it into the prepared 37°C water bath, and shake it gently to dissolve it quickly. Then, after wiping with an alcohol cotton ball, move it into the ultra-clean workbench. Use a pipette gun to put the aspirated cell mixture into a centrifuge tube. After centrifugation, remove the supernatant, resuspend the medium, blow off the ...

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PUM

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Abstract

The invention relates to the technical field of medicine, in particular to a composition of Tenacissoside H and cis-platinum and application thereof. According to the composition, a molar concentration ratio of Tenacissoside H to cis-platinum is 1: 2. According to the invention, human lung cancer cells A549, esophageal cancer cells KYSE70 and ovarian cancer cells SK-OV-3 are used as in-vitro experimental models, and cell proliferation inhibition effect of combined administration of Tenacissoside H and cis-platinum is observed. Experimental results show that compared with a control group (a single administration group), the combined administration group has obvious inhibition on cell proliferation, can significantly enhance effects of chemotherapeutic drugs in tumor treatment, and has prospect in clinical combined administration.

Description

technical field [0001] The invention relates to the field of technical medicine, in particular to the composition of guanine glucoside H and cisplatin and its application. Background technique [0002] Malignant tumors—cancer has become one of the major diseases that are common and seriously threaten human life and quality of life. In recent years, with the development of economy and society, although people's living standards are constantly improving, the morbidity and mortality of cancer are constantly increasing. [0003] The Western medicines currently used clinically to treat tumors have large toxic and side effects, and cause great harm to the body of tumor patients during the treatment. In addition, there are also problems such as decreased sensitivity of malignant tumor cells to Western medicines. It makes the disease more and more difficult to treat, and the quality of life of patients decreases. [0004] Apoptosis refers to the process in which internal and exter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/243A61K31/7048A61P35/00
CPCA61K33/243A61K31/7048A61P35/00A61K2300/00
Inventor 胡道德王恺纯
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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