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Genetic marker for evaluating quality of bovine semen and application of genetic marker

A genetic marker and semen technology, applied in the field of genetic markers to evaluate the quality of bovine semen, can solve the problems of no bovine semen kit, no semen quality traits, etc.

Active Publication Date: 2021-07-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no research report on whether FHL2 affects semen quality traits, and there is no kit that can be used to evaluate bovine semen quality

Method used

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  • Genetic marker for evaluating quality of bovine semen and application of genetic marker
  • Genetic marker for evaluating quality of bovine semen and application of genetic marker
  • Genetic marker for evaluating quality of bovine semen and application of genetic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Obtaining of bovine FHL2 gene promoter region DNA fragment and establishment of SNP detection method

[0021] The present invention has gone through such as figure 1 The shown process and technical analysis have obtained the bovine FHL2 gene promoter sequence fragment, the fragment length is 1564bp, and its nucleotide sequence is as described in the sequence table SEQ ID NO.1, by performing blast comparison on the above sequence on the NCBI website, in A nucleotide polymorphism (SNP) site is found in the amplified fragment.

[0022] According to bovine FHL2 genome sequence (NCBI reference sequence: NC_037338.1) design following primer pair, concrete sequence is as follows:

[0023] Forward primer 1 (SEQ ID NO.2): 5'-GGGCTTAAAGAACGTGTGTGTG-3',

[0024] Reverse primer 1 (SEQ ID NO.3): 5'-TGACCACCTAGGTCTGGAATGA-3'.

[0025] Forward primer 2 (SEQ ID NO.4): 5'-CATTCCAGACCTAGGTGGTCATT-3',

[0026] Reverse primer 2 (SEQ ID NO.5): 5'-AGGCAAAGCCCTGTATCCAA-3'.

[0...

Embodiment 2

[0036] Example 2: Association analysis of genetic markers of the present invention and bovine semen quality traits

[0037] In order to determine whether the SNP in the promoter region of the bovine FHL2 gene is related to the difference in bovine phenotype, a total of 246 cattle of different breeds (Holstein and Simmental) were selected as test materials. The T879G polymorphic site was genotyped by the first-generation sequencing method, and the specific method was as follows: PCR amplification was performed on the genomic DNA extracted from the semen of 246 cattle with the primer pair shown in SEQ ID NO.4 and SEQ ID NO.5. (Amplification adopts the conditions in Example 1, and the fragment of the amplified product should be the fragment shown in the 648bp-1170bp in SEQID NO.1), after the amplified product is detected by 2% agarose gel electrophoresis, Commissioned Wuhan Tianyi Huiyuan Biotechnology Co., Ltd. for sequencing.

[0038] Three genotypes were detected in this popu...

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PUM

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Abstract

The invention belongs to the technical field of preparation of genetic markers of cattle, and particularly relates to a genetic marker associated with semen quality traits, the marker is screened from a cattle FHL2 gene, the sequence of the marker is a 648bp-1170bp nucleotide sequence as shown in SEQ ID NO.1, T / G base mutation occurs at the 879th base r of the sequence, and FHL2 gene polymorphism is caused. The invention discloses an SNP typing detection technology of a promoter region of the bovine FHL2 gene, and finds that a T / G mutation site at the 879th basic group as shown in SEQ ID NO.1 is obviously related to the average sampling quantity character. The invention provides a new genetic marker for cattle reproductive trait marker-assisted selection.

Description

technical field [0001] The invention belongs to the technical field of marker-assisted selection of cattle, and in particular relates to a genetic marker for evaluating bovine semen quality and its application. Background technique [0002] With the widespread use of frozen semen and estrus-timed insemination techniques, the reproductive ability of high-quality bulls has been greatly demonstrated, and their genetic improvement capabilities have also been brought into play. A large number of research data show that the pros and cons of breeding bulls are crucial to the genetic improvement of dairy cattle populations, which account for 70% of the progress of genetic improvement of dairy cattle populations. With the development of molecular biology, the emergence of molecular marker technology makes up for the shortcomings of traditional breeding methods based on phenotype selection, and promotes the efficiency and progress of breeding. [0003] The research and application of...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 滑国华张燊孙晖邵宝顺曹家瑄杜超林雨昕
Owner HUAZHONG AGRI UNIV
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