Pulmonary fibrosis cynomolgus monkey model induced by bleomycin and application of cynomolgus monkey model
A technology for bleomycin and pulmonary fibrosis, which is applied in the field of experimental animals and can solve the problem of the distant relationship between animal models and humans
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Embodiment 1
[0044] Example 1 Breeding of experimental animals.
[0045] Ordinary adult male cynomolgus monkeys, weighing 4-6 kg, aged 5-7 years. The growth environment is 12 h / 12 h day and night cycle, the humidity is 45% to 70%, and the temperature is 18°C to 26°C. Humane care was given according to the 3R principle of experimental animals, and relevant physiological and biochemical indicators were monitored before the experiment to ensure the health of experimental animals.
Embodiment 2
[0046] Example 2 Anesthesia and endotracheal tube insertion in cynomolgus monkeys.
[0047] The cynomolgus monkey was anesthetized with 5-10mg / kg Shutai, lying supine on the operating table, tilting the head back, inserting the laryngoscope in the left hand along the curvature of the back of the tongue to the root of the tongue, lightly provoking the epiglottis cartilage, exposing the glottis, and opening the glottis , the right hand holding a suitable size endotracheal tube quickly inserted into the trachea and out of the laryngoscope. Check whether there is gas discharged from the outer port of the endotracheal tube with breathing and whether the breath sounds at the apices of the two lungs are consistent during auscultation. After confirming that the intubation is correct, inject an appropriate amount of air into the catheter balloon to fix the catheter. A cynomolgus monkey weighing about 4kg has a respiratory frequency of about 18-25 and a tidal volume of about 40-80ml.
Embodiment 3
[0048] Example 3 Treatment of control cynomolgus monkeys (atomized saline inhalation).
[0049] After the cynomolgus monkey was anesthetized according to the method in Example 2, a tracheal tube was inserted.
[0050] The endotracheal tube is connected to the Y-shaped head, and one end of the Y-shaped head is connected to the Yuwell nebulizer (the atomization speed of the nebulizer is about 0.15 ml / min), the air flow rate is 1L / min, and the liquid concentration in the nebulized gas is 0.0125-0.5 ml / L / min. The other end of the Y-shaped head is connected to the exhaust gas recovery device. Add the nebulizer to nebulize the physiological saline in the container. A cynomolgus monkey weighing about 4kg has a respiratory frequency of about 18 and a tidal volume of about 40ml. Turn on the atomizer switch, and the cynomolgus monkeys keep inhaling the mist for 10 minutes.
[0051] For the treated cynomolgus monkeys, bronchoscopy and CT imaging scans were performed every week, and n...
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