A cynomolgus monkey model of bleomycin-induced pulmonary fibrosis and its application

A technology of bleomycin and pulmonary fibrosis, applied in the field of experimental animals, can solve the problem of distant relationship between animal models and humans

Active Publication Date: 2022-02-22
澎立生物医药技术(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to solve the shortage of drugs for treating pneumonia fibrosis in the prior art, and the problem that the animal models for screening such drugs are far from human relatives, and provide an animal model of pulmonary fibrosis that is closer to human relatives

Method used

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  • A cynomolgus monkey model of bleomycin-induced pulmonary fibrosis and its application
  • A cynomolgus monkey model of bleomycin-induced pulmonary fibrosis and its application
  • A cynomolgus monkey model of bleomycin-induced pulmonary fibrosis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Breeding of experimental animals.

[0045] Ordinary adult male cynomolgus monkeys, weighing 4-6 kg, aged 5-7 years. The growth environment is 12 h / 12 ​​h day and night cycle, the humidity is 45% to 70%, and the temperature is 18°C ​​to 26°C. Humane care was given according to the 3R principle of experimental animals, and relevant physiological and biochemical indicators were monitored before the experiment to ensure the health of experimental animals.

Embodiment 2

[0046] Example 2 Anesthesia and endotracheal tube insertion in cynomolgus monkeys.

[0047] The cynomolgus monkey was anesthetized with 5-10mg / kg Shutai, lying supine on the operating table, tilting the head back, inserting the laryngoscope in the left hand along the curvature of the back of the tongue to the root of the tongue, lightly provoking the epiglottis cartilage, exposing the glottis, and opening the glottis , the right hand holding a suitable size endotracheal tube quickly inserted into the trachea and out of the laryngoscope. Check whether there is gas discharged from the outer port of the endotracheal tube with breathing and whether the breath sounds at the apices of the two lungs are consistent during auscultation. After confirming that the intubation is correct, inject an appropriate amount of air into the catheter balloon to fix the catheter. A cynomolgus monkey weighing about 4kg has a respiratory frequency of about 18-25 and a tidal volume of about 40-80ml.

Embodiment 3

[0048] Example 3 Treatment of control cynomolgus monkeys (atomized saline inhalation).

[0049] After the cynomolgus monkey was anesthetized according to the method in Example 2, a tracheal tube was inserted.

[0050] The endotracheal tube is connected to the Y-shaped head, and one end of the Y-shaped head is connected to the Yuwell nebulizer (the atomization speed of the nebulizer is about 0.15 ml / min), the air flow rate is 1L / min, and the liquid concentration in the nebulized gas is 0.0125-0.5 ml / L / min. The other end of the Y-shaped head is connected to the exhaust gas recovery device. Add the nebulizer to nebulize the physiological saline in the container. A cynomolgus monkey weighing about 4kg has a respiratory frequency of about 18 and a tidal volume of about 40ml. Turn on the atomizer switch, and the cynomolgus monkeys keep inhaling the mist for 10 minutes.

[0051] For the treated cynomolgus monkeys, bronchoscopy and CT imaging scans were performed every week, and n...

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Abstract

The invention relates to the establishment of a cynomolgus monkey animal model of pulmonary fibrosis. Including the joint operation of nebulized inhalation of bleomycin and spraying bleomycin to the tracheal carina of cynomolgus monkeys. On the basis of the traditional nebulized inhalation operation, the operation of spraying bleomycin is further added. The method of the invention is simple to operate and does not cause any trauma to the cynomolgus monkey. The method of the invention is used to successfully obtain an animal model of pulmonary fibrosis in the cynomolgus monkey, and the pulmonary fibrosis index of the cynomolgus monkey model is remarkable. The invention establishes an animal model of pulmonary fibrosis with a more ideal human relative. The prepared animal model can be further applied to the preparation of drugs for treating pulmonary fibrosis.

Description

technical field [0001] The invention relates to the field of experimental animals, in particular to a cynomolgus monkey model of pulmonary fibrosis induced by bleomycin and its application. Background technique [0002] Experimental animals are ideal tools for crossing, penetrating and synthesizing related disciplines. The establishment of ideal animal models of human diseases provides reliable and useful means and advanced tools for research in various fields of life sciences such as medicine, pharmacy and genetics. In the research fields of tumor immunity, organ transplant rejection immunity, clinical immunity and other immune-related diseases, as well as various infectious diseases, immunosuppressive animal models are indispensable and key tools. How to successfully construct Immunosuppressive animal models have become an urgent problem to be solved. [0003] At present, the research data of immunosuppressive animal models at home and abroad are mainly based on rodents ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2227/106A01K2267/03
Inventor 杨平戴飞红俞诚段继峰
Owner 澎立生物医药技术(上海)股份有限公司
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