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Application of Vc derivative in promoting erythrocyte regeneration and product

A technology of erythrocytes and derivatives, applied in the field of biomedicine, can solve the problems of retention, erythrocyte proliferation, and low efficiency of induced differentiation, and achieve the effects of increasing 5hmC levels, promoting erythroid differentiation, and increasing protein levels

Inactive Publication Date: 2021-07-02
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on vitamin C magnesium phosphate is still in the additive stage, which is mainly used in skin care products or feed additives, and uses its characteristics of eliminating oxygen free radicals to play wrinkle-removing and anti-aging effects; while for vitamin C Other uses of magnesium phosphate are rarely reported
[0005] Due to the shortage of blood sources and the occurrence of blood transfusion-related diseases, people's demand for blood sources, and the existing problems of low red blood cell proliferation and induction differentiation efficiency, the present invention proposes the application of Vc derivatives in promoting red blood cell regeneration to improve red blood cell regeneration in vitro Efficiency, providing new ideas for the treatment of anemia

Method used

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  • Application of Vc derivative in promoting erythrocyte regeneration and product
  • Application of Vc derivative in promoting erythrocyte regeneration and product
  • Application of Vc derivative in promoting erythrocyte regeneration and product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1Vc

[0047] Example 1 Vc derivatives promote CD34 derived from human umbilical cord blood + Erythroid differentiation

[0048] S1: Prepare Vc magnesium phosphate to 100mM, according to the final concentration of 100μM, that is, dilute 1000 times and add to CD34 + In the cell induction differentiation system, it was added on the 3rd day of differentiation, and then replaced with a new medium containing the same concentration of vitamin C at the time point of differentiation.

[0049] S2: 100,000 cells on day 7 were incubated with CD71 and CD235 flow antibodies for 10 minutes, and the residual antibodies in the solution were washed away for flow analysis.

[0050] Such as figure 1 As shown, the results showed that in CD34 + In the cell differentiation system, the treatment of Vc derivatives can significantly promote the differentiation of erythrocytes compared with the control group (untreated).

Embodiment 2V

[0051] Example 2 Vc derivatives promote CD34 derived from human umbilical cord blood + Cell differentiation system and proliferation of HUDEP2 erythroid lineage erythroid differentiation

[0052] S1: Prepare Vc magnesium phosphate to 100mM, according to the final concentration of 100μM, that is, dilute 1000 times and add to CD34 + In the cell induction differentiation system, it was added on the 3rd day of differentiation, and then replaced with a new medium containing the same concentration of vitamin C at the time point of differentiation.

[0053] Add it to the HUDEP2 erythroid induction differentiation system according to the required concentration, add it on the 0th day of differentiation, and then replace the new medium containing vitamin C at the differentiation time point, the replacement time point is the 4th day, and differentiate to the 7th day .

[0054] S2: Plant the treated two kinds of cells in a 60mm culture dish at a density of 1-2million / ml respectively, CD...

Embodiment 3V

[0058] Example 3 Vc derivatives promote CD34 derived from human umbilical cord blood + Expression of erythroid differentiation-related genes in erythroid cells and HUDEP2 erythroid-induced differentiation system

[0059] S1: with the S1 step in embodiment 2.

[0060] S2: with the S2 step in embodiment 2.

[0061] S3: Take the 7th and 10th day of differentiation, extract the RNA of the cells by Trizol reagent, isopropanol, and 75% absolute ethanol, and complete the reverse transcription of RNA according to the reverse transcription steps of the TAKARA reverse transcription kit to obtain cDNA. KAPA SYBRFAST Universal qPCR Kit completes qPCR.

[0062] The results show that if Figure 3-7 As shown, in CD34 + In the cell-induced differentiation system and HUDEP2 erythroid-induced differentiation, compared with the control group (untreated), Vc derivative treatment can significantly promote erythroid differentiation-related genes at different differentiation time points, such as...

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Abstract

The invention discloses application of a Vc derivative in promoting erythrocyte regeneration and a product, and belongs to the field of biological medicine. The invention provides an application of a Vc (vitamin C) derivative in promoting red blood cell regeneration and a product, by continuously adding a certain dose of the Vc derivative into a culture solution, erythroid differentiation can be promoted, the regeneration efficiency of an erythroid in-vitro differentiation system is improved, the cell proliferation capacity is improved, and the expression of related genes of erythroid differentiation is improved; the in-vitro erythrocyte regeneration efficiency is improved, and a new thought is provided for treating anemia.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application and product of a Vc derivative in promoting red blood cell regeneration. Background technique [0002] Vitamin C, also known as ascorbic acid, has the molecular formula C6H8O6 (Ascorbate). As we all know, vitamin C deficiency mainly causes bleeding and bone lesions clinically, and those who are severely deficient will suffer from anemia. There are two possible factors for the formation of vitamin C deficiency anemia, one is the anemia caused by the skin, deep tissue and even gastrointestinal bleeding of patients with vitamin C deficiency; the second is that vitamin C participates in the metabolism of iron and folic acid, which can make the trivalent Iron is reduced to easily absorbed ferrous iron, thereby promoting the absorption of iron, and making sulfhydryl groups such as ferrous complexing enzymes active, most of which will be used in the synthesis of he...

Claims

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Application Information

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IPC IPC(8): C12N5/078C12N5/0789A61K31/375A61P7/06
CPCA61K31/375A61P7/06C12N5/0641C12N5/0647C12N2500/38
Inventor 方向东张昭军辛子娟谭肖喻阮修艳
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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