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Method for establishing grass carp intestinal flora imbalance model

A technology for intestinal flora imbalance and method establishment, which can be applied in fish farming, pharmaceutical formulations, medical preparations containing active ingredients, etc., and can solve the problems that there are no studies describing the effects of antibiotics on the intestinal flora of grass carp

Pending Publication Date: 2021-06-29
SUZHOU UNIV
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  • Application Information

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Problems solved by technology

Although antibiotics are frequently used in aquaculture of this species, no studies have described the effects of antibiotics on the gut microbiota of grass carp
[0004] Currently, little is known about the interplay between pathogenic and beneficial microbes in the pathogenic bacterial community, the causal relationship between enterobacteriosis and disease, although we know the effects of antibiotics on gut microbiota and fish health

Method used

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  • Method for establishing grass carp intestinal flora imbalance model
  • Method for establishing grass carp intestinal flora imbalance model
  • Method for establishing grass carp intestinal flora imbalance model

Examples

Experimental program
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Embodiment Construction

[0016] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0017] 1. Specific examples

[0018] 1. Experimental fish: The two-year-old grass carp used in the experiment was purchased from Future Aquaculture Farm (Suzhou, China). Before the study, the experimental fish had not used any antibiotics. In the aquaculture system of Soochow University, the fish were placed in 18 culture tanks (500L) for two weeks under the condition of continuous aeration and oxygenation. Fish were fed commercial feed from Shanghai Zhongwang Feed Co., Ltd. (Shanghai, China) at 2% of body weight twice a day at 8:30 and 16:30. During the experiment, the water temperature and pH were kept at 27±2°C and 7.2±0.4 respectively, the dissolved oxygen in the water was greater than 6.0mg / L, and about one-third of the water was renewed every day.

[0019] 2. Antibiotic treatment: After adaptation, a total of 180 healthy fish (53 ± 4g...

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Abstract

The invention relates to a method for establishing a grass carp intestinal flora imbalance model. The method for establishing the grass carp intestinal flora imbalance model is characterized in that grass carp is used as an experimental animal and is fed with mixed antibiotics for 28 days, and the antibiotics are prepared from 2g / kg of florfenicol, 2g / kg of enrofloxacin, 2g / kg of metronidazole and 1g / kg of vancomycin. The method for establishing the grass carp intestinal flora imbalance model has the advantages that an effective tool is provided for researching the pathogenesis of bacterial enteritis and screening effective medicines and beneficial microorganisms for treating enteritis.

Description

technical field [0001] The invention relates to a method for establishing an animal experiment model, in particular to a method for establishing a grass carp intestinal flora imbalance model. Background technique [0002] Gut microbiota plays a key role in regulating host immunity and maintaining immune homeostasis, and it is widely accepted that a stable gut microbiota structure is a key factor in maintaining fish health. Previous studies have shown that nutritional imbalances, physiological changes, and immunocompromised immune function are associated with altered gut microbiota composition and diversity due to dietary oxidized fish oils, high-fat diets, environmental pollutants, high-salt stress, exposure to antibiotics and bacteria related. These adverse factors have been unequivocally associated with intestinal dysbiosis, which is characterized by changes in the composition and diversity of the gut microbiota. Among the above factors, the use of antibiotics is conside...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A23K50/80A23K20/195A61K31/165A61K31/496A61K31/4164A61K38/14
CPCA01K61/10A23K50/80A23K20/195A61K31/165A61K31/496A61K31/4164A61K38/14A01K2267/03A01K2227/40Y02A40/81
Inventor 宋学宏孙丙耀杨会兴何稳田丹阳
Owner SUZHOU UNIV
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