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Method for detecting genetic and epigenetic changes in tumor stem cells

A cancer stem cell and epigenetic technology, applied in the field of detecting genetic and epigenetic changes in cancer stem cells, can solve problems such as the difficulty of studying human CSCs

Pending Publication Date: 2021-06-25
THE NAVAL MEDICAL UNIV OF PLA
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Problems solved by technology

However, studying human CSCs has always been difficult because they usually constitute only a small fraction of clinical patient tumor specimens

Method used

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  • Method for detecting genetic and epigenetic changes in tumor stem cells
  • Method for detecting genetic and epigenetic changes in tumor stem cells
  • Method for detecting genetic and epigenetic changes in tumor stem cells

Examples

Experimental program
Comparison scheme
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Embodiment

[0053] 1. Materials and methods

[0054] 1. Collection of Clinical Specimens

[0055] Fresh primary liver cancer tissues and paired paracancerous tissues were obtained from patients undergoing surgical resection at Shanghai Oriental Hepatobiliary Surgery Hospital (EHBH). Each tissue specimen was embedded in OCT, then immediately frozen in isopentane precooled with liquid nitrogen, and finally stored in a -80°C freezer until further processing. Following surgical resection, each specimen for cryosection was embedded within 30 minutes. All patient files were collected through the EHBH file system, informed consent was obtained from the patients, and all procedures were approved by the EHBH ethics committee.

[0056] 2. Immunohistochemistry (IHC)

[0057] Briefly, for cryosection IHC, OCT-embedded frozen tissues were cut into 8 μm thick sections; 2 o 2inactivated by incubation. Nonspecific signal was blocked using 5% bovine serum albumin. The primary antibodies used were a...

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Abstract

The invention relates to the technical field of biology, in particular to a method for detecting genetic and epigenetic changes in tumor stem cells and clinical application. It is found that compared with para-carcinoma tissue, although wide genetic and epigenetic changes exist in liver cancer tissue, only a small amount of key genetic and epigenetic changes exist in liver cancer stem cells in the liver cancer tissue. For treatment of key genetic and epigenetic changes occurring in the tumor stem cells, the treatment effect on cancers may be better. The invention provides a new target discovery method for treatment of liver cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting genetic and epigenetic changes in tumor stem cells and its clinical application. Background technique [0002] Cancer stem cells (CSCs) make up a very small proportion of the tumor cell population, but they play a key role in tumorigenesis. A fundamental question in tumor biology is which genetic and epigenetic changes occur in CSCs and whether these changes are similar to those in other tumor parenchymal cells of that tumor. [0003] Carcinogenesis is a complex evolutionary process. We now know that tumor cells undergo significant genetic and epigenetic changes for many reasons compared with normal cells. Many cancers are thought to originate from CSCs, which confer resistance to tumor therapy and a high risk of recurrence. Understanding genetic and epigenetic changes in human CSCs can provide inspiration for designing new and better cancer treatments. Howe...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6869
CPCC12Q1/6886C12Q1/6869C12Q2600/154C12Q2531/119C12Q2537/143C12Q2523/125C12Q2523/319
Inventor 贺宇飞王红阳
Owner THE NAVAL MEDICAL UNIV OF PLA
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