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Efficient amino acid fermentation liquor low-temperature pressurization sterilization method

A sterilization method and fermentation broth technology, applied in the biological field, can solve the problems of increasing the difficulty of purification treatment, affecting the purity and appearance of L-valine, and achieve the effects of being beneficial to decolorization treatment, low sterilization temperature and cost reduction.

Pending Publication Date: 2021-06-25
ANHUI HUAHENG BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under high temperature conditions, complex chemical reactions (such as Maillard reaction) are prone to occur in the fermentation broth, producing pigments and other impurity components, which increases the difficulty of subsequent purification and affects the purity and appearance of L-valine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of L-valine Fermentation Sterilization Solution by Low Temperature and Pressure Sterilization

[0033] The low-temperature pressurized sterilization method prepares the L-valine fermentation sterilization liquid, comprises the following steps:

[0034] (1) 1 mL of Brevibacterium flavum was inoculated into 50 mL of LB medium, and cultured on a shaker at 37°C and 210 r / min until pH 6.2-6.5 and OD value (absorbance) was 3-4, and obtained first-class seed liquid;

[0035] (2) Take 2mL of primary seed liquid, inoculate it into 100mL of synthetic medium, and then culture it on a shaking table at 37°C and 210r / min until pH6.4-6.6 and OD value is 3- 4. Obtain L-valine seed culture solution;

[0036] (3) Inoculate the L-valine seed culture solution into the fermentation medium according to the inoculation amount of 2%, and place it at 35°C and pH6-7 for fermentation and cultivation until the residual sugar content is not higher than 0.5g / L , collecting ...

Embodiment 2

[0039] Example 2 Preparation of L-valine Fermentation Sterilization Solution by Low Temperature and Pressure Sterilization

[0040] The low-temperature pressurized sterilization method prepares the L-valine fermentation sterilization liquid, comprises the following steps:

[0041] (1) Inoculate 1 mL of Brevibacterium flavum into 50 mL of LB medium, culture it on a shaker at 37°C and 210 r / min until pH 6.2-6.5, OD value 3-4, and obtain the first-grade seed liquid ;

[0042] (2) Take 2mL of primary seed liquid, inoculate it into 100mL of synthetic medium, and then culture it on a shaking table at 37°C and 210r / min until pH6.4-6.6 and OD value is 3- 4 Terminate the cultivation to obtain the L-valine seed culture solution;

[0043] (3) Inoculate the L-valine seed culture solution into the fermentation medium according to the inoculation amount of 2%, and place it at 35°C and pH6-7 for fermentation and cultivation until the residual sugar content is not higher than 0.5g / L , co...

Embodiment 3

[0046] Example 3 Preparation of L-valine Fermentation Sterilization Solution by Low Temperature and Pressure Sterilization

[0047] The low-temperature pressurized sterilization method prepares the L-valine fermentation sterilization liquid, comprises the following steps:

[0048] (1) Inoculate 1 mL of Brevibacterium flavum into 50 mL of LB medium, culture it on a shaker at 37°C and 210 r / min until pH 6.2-6.5, OD value 3-4, and obtain the first-grade seed liquid ;

[0049] (2) Take 2mL of primary seed liquid, inoculate it into 100mL of synthetic medium, and then culture it on a shaking table at 37°C and 210r / min until pH6.4-6.6 and OD value is 3- 4. Obtain L-valine seed culture solution;

[0050] (3) Inoculate the L-valine seed culture solution into the fermentation medium according to the inoculation amount of 2%, and then place it at 35°C and pH6-7 for fermentation and cultivation until the residual sugar content is not higher than 0.5g / L, collecting L-valine fermentatio...

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Abstract

The invention relates to an efficient amino acid fermentation liquor low-temperature pressurization sterilization method, which comprises the following steps of: sterilizing an amino acid fermentation liquor under the conditions that the pressure is 0.1-0.4 MPa and the temperature is not higher than 80 DEG C to prepare the amino acid fermentation sterilization liquor. According to the sterilization method, the viable count is 0, the sterilization temperature is low, the energy consumption is low, the light transmittance of the sterilization fermentation liquid is improved, the pigment content in the fermentation sterilization liquid is reduced, decolorization treatment is facilitated, the cost is remarkably reduced, and the sterilization method is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a high-efficiency low-temperature pressurization method for amino acid fermentation broth. Background technique [0002] The method of preparing amino acids by microbial fermentation has the advantages of bio-based raw materials, high-efficiency conversion, mild reaction, environmental protection and economy, and has been widely used in the industry. The method uses glucose as a raw material to obtain amino acid fermentation liquid through microbial fermentation. Amino acid products are obtained from the fermentation broth after sterilization, decolorization, concentration, crystallization, separation and other treatments. [0003] Sterilization is one of the necessary processing steps of fermentation broth. Sterilization denatures and coagulates the bacterial protein in the fermentation broth to terminate the reaction, avoiding the leakage of live bacteria and phage infection. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12N1/20A61L2/04A61L2/02C12R1/13
CPCC12P13/08C12N1/20A61L2/0023A61L2/0011
Inventor 余军刘树蓬刘磊张大伟马祥亮
Owner ANHUI HUAHENG BIOTECH
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