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Primer probe composition, kit and method for detecting mycobacterium tuberculosis and nontuberculous mycobacterium

A technology of mycobacterium tuberculosis and primer probes, which is applied in the biological field, can solve the problems of difficulty in accurately distinguishing mycobacterium tuberculosis from non-tuberculosis mycobacteria, insufficient sensitivity, cumbersome operation, etc., and achieve rapid and sensitive detection, probe well-designed effects

Pending Publication Date: 2021-06-22
PILOT GENE TECH HANGZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The disadvantages of the existing technology mainly include that the operation is relatively cumbersome and time-consuming, the sensitivity is not enough, and it is difficult to accurately distinguish tuberculosis mycobacteria from non-tuberculous mycobacteria, etc.

Method used

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  • Primer probe composition, kit and method for detecting mycobacterium tuberculosis and nontuberculous mycobacterium
  • Primer probe composition, kit and method for detecting mycobacterium tuberculosis and nontuberculous mycobacterium
  • Primer probe composition, kit and method for detecting mycobacterium tuberculosis and nontuberculous mycobacterium

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Embodiment 1

[0035] This embodiment discloses a method for simultaneously detecting tuberculosis mycobacteria and non-tuberculous mycobacteria.

[0036] 1. Experimental materials:

[0037] 1. Sample requirements:

[0038] 1.1 Mycobacterium tuberculosis DNA: positive DNA detected by Xpert;

[0039] 1.2 Nontuberculous Mycobacterium DNA: Mycobacterium avium DNA verified by next-generation sequencing;

[0040] 1.3 Normal human DNA: genomic DNA extracted from oral swabs;

[0041] 1.4 Bacterial DNA: DNA extracted from inactivated positive strains purchased by our company.

[0042] 2. Experimental flow chart:

[0043] Sample extraction of free nucleic acid→configuration of digital PCR reaction solution→droplet chip generation→amplification→reading→result analysis and report output

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Abstract

The invention belongs to the field of biology, and particularly relates to a primer probe composition, a kit and a method for detecting mycobacterium tuberculosis and nontuberculous mycobacterium. The invention discloses a primer probe composition. The primer probe composition comprises a nucleotide sequence group with nucleotide sequences as shown in SEQ ID NO: 1-6. The invention also discloses a kit comprising the primer probe composition, and a method for detecting mycobacterium tuberculosis and nontuberculous mycobacterium by using the primer probe composition or the kit. The kit disclosed by the invention is based on a multiple droplet digital PCR platform, and the platform has the characteristics of high sensitivity and multiple applicability. In addition, through combination optimization of the autonomous geophysical probes, a reasonable geophysical probe pair combination is screened out; therefore, the composition can be used for rapidly and sensitively detecting the mycobacterium tuberculosis and the nontuberculous mycobacterium.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a primer probe composition, a kit and a method for detecting mycobacterium tuberculosis and non-tuberculosis mycobacteria. Background technique [0002] Mycobacterium tuberculosis (M.tuberculosis), commonly known as Mycobacterium tuberculosis, is the pathogenic bacteria that causes tuberculosis. It can invade all organs of the body, but tuberculosis is the most common. Tuberculosis is still an important infectious disease. According to the WHO, about 8 million new cases occur each year and at least 3 million people die from the disease. Before the founding of the People's Republic of my country, the mortality rate reached 200-300 people / 100,000, ranking first among the causes of death from various diseases. Morbidity and mortality are greatly reduced. However, it should be noted that in some areas of the world, the incidence rate is on the rise again due to AIDS, drug abuse,...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/32
CPCC12Q1/689C12Q1/686C12Q2537/143C12Q2563/159C12Q2563/107
Inventor 朱海涛夏江
Owner PILOT GENE TECH HANGZHOU CO LTD
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