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Radix gentianae compound enzyme as well as preparation method and application thereof

A compound and enzyme technology, applied in the field of gentian compound enzyme and its preparation, can solve problems such as poor use effect and low content of effective substances, and achieve the effects of improving skin condition, reducing toxicity and clarifying the mechanism of drug action

Active Publication Date: 2021-06-22
GUANGDONG DIMEI NEW MATERIAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The active substance content of the product obtained through fermentation is low, and the effect is not good after being added to daily chemical products

Method used

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  • Radix gentianae compound enzyme as well as preparation method and application thereof
  • Radix gentianae compound enzyme as well as preparation method and application thereof
  • Radix gentianae compound enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the gentian compound ferment of the present embodiment comprises the following steps:

[0034] 1. Weigh 10 parts of fresh gentian, 5 parts of watercress, and 5 parts of Centella asiatica by weight, crush them, pass through an 80-mesh sieve, add deionized water 3 times the weight of the powder to dissolve, and prepare a solution ;

[0035] 2. Add 0.5% cellulase and 0.5% pectinase to the solution, and treat for 60-120 minutes to obtain an enzymatic solution;

[0036] 3. Add 1% Yeast Extract (yeast extract), 2% Peptone (peptone), 2% Dextrose (glucose) (glucose) of the mass of the enzymolysis solution to the enzymolysis solution and transfer to a bioreactor for sterilization at 115°C for 15 minutes. Prepare a sterilizing solution;

[0037] 4. In the sterilization solution, according to the strain combination of Saccharomyces cerevisiae and Lactobacillus plantarum 1:1, the inoculation amount is 10% to insert the strains

[0038] 5. Set the fermen...

Embodiment 2

[0040] The preparation method of the gentian compound ferment of the present embodiment comprises the following steps:

[0041] 1. Take 8 parts by weight of fresh gentian, 4 parts of watercress, and 4 parts of Centella asiatica, crush them, pass through an 80-mesh sieve, add deionized water 3 times the weight of the powder to dissolve, and prepare a solution ;

[0042]2. Add 0.5% cellulase and 0.5% pectinase to the solution, and treat for 60-120 minutes to obtain an enzymatic solution;

[0043] 3. Add 1% Yeast Extract (yeast extract), 2% Peptone (peptone), 2% Dextrose (glucose) (glucose) of the mass of the enzymolysis solution to the enzymolysis solution and transfer to a bioreactor for sterilization at 115°C for 15 minutes. Prepare a sterilizing solution;

[0044] 4. In the sterilization solution, according to the strain combination of Saccharomyces cerevisiae and Lactobacillus plantarum 1:1, the inoculation amount is 10% to insert the strains

[0045] 5. Set the fermentat...

Embodiment 3

[0047] The preparation method of the gentian compound ferment of the present embodiment comprises the following steps:

[0048] 1. Weigh 6 parts of fresh gentian, 5 parts of watercress, and 5 parts of Centella asiatica by weight, crush them, pass through an 80-mesh sieve, add deionized water 3 times the weight of the powder to dissolve, and obtain a solution ;

[0049] 2. Add 0.5% cellulase and 0.5% pectinase to the solution, and treat for 60-120 minutes to obtain an enzymatic solution;

[0050] 3. Add 1% Yeast Extract (yeast extract), 2% Peptone (peptone), 2% Dextrose (glucose) (glucose) of the mass of the enzymolysis solution to the enzymolysis solution and transfer to a bioreactor for sterilization at 115°C for 15 minutes. Prepare a sterilizing solution;

[0051] 4. In the sterilization solution, according to the strain combination of Saccharomyces cerevisiae and Lactobacillus plantarum 1:1, the inoculation amount is 10% to insert the strains

[0052] 5. Set the fermenta...

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Abstract

The invention discloses a radix gentianae compound enzyme preparation method, which comprises: S10, selecting fresh radix gentianae, swertia and centella asiatica, crushing, screening, and adding deionized water to dissolve the screened powder so as to obtain a dissolving solution; S20, adding cellulase and pectinase into the dissolving solution for enzymolysis treatment, and obtaining enzymatic hydrolysate; S30, adding yeast extract, peptone and glucose into the enzymatic hydrolysate, and then performing sterilization treatment to obtain a sterilized solution; and S40, inoculating a strain combination consisting of saccharomyces cerevisiae and lactobacillus plantarum into the sterilized solution, and performing fermentation treatment to obtain the radix gentianae compound enzyme. The radix gentianae compound enzyme prepared by the method disclosed by the invention is high in content of effective substances, can be applied to daily chemical products to strengthen skin functions and improve skin conditions, and has very good anti-allergy soothing and anti-oxidation effects.

Description

technical field [0001] The invention relates to the technical field of daily chemical products, in particular to a gentian compound enzyme and its preparation method and application. Background technique [0002] As people pay more attention to healthy life and the environment, the side effects of chemically synthesized raw materials are increasing consumers' concerns, and daily chemical products are more and more advocating natural. At present, most of the plant raw materials used in domestic daily chemical products are added in the form of extracts. Due to the limitation of the process, the content of active ingredients in the extracts is low, and the efficacy cannot be guaranteed. For extraction, the appropriate solvent is generally selected according to the polarity of the active ingredients of the plant. For components with high polarity, water extraction is generally used. For components with low polarity, alcohol extraction is generally used. Generally, propylene glyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08A61Q19/00
CPCA61K2800/5922A61K2800/805A61K2800/85A61Q19/005A61Q19/08A61K8/9789
Inventor 陶宏兵刘永龙林家洪杨娟邓小锋周刚李文茹谢小保
Owner GUANGDONG DIMEI NEW MATERIAL TECH CO LTD
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