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Monoclonal antibody for identifying anti-CLDN18_2 antibody and preparation method and application thereof

A monoclonal antibody and antibody technology, applied in the biological field, can solve the problem of no detection antibody, and achieve the effect of huge market value and broad application prospects.

Active Publication Date: 2021-06-18
DRAGONBOAT BIOPHARMACEUTICAL (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the NA1-S antibody has broad prospects for development, but due to its novelty, there is currently no effective means to detect this antibody in blood

Method used

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  • Monoclonal antibody for identifying anti-CLDN18_2 antibody and preparation method and application thereof
  • Monoclonal antibody for identifying anti-CLDN18_2 antibody and preparation method and application thereof

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Embodiment 1

[0059] This embodiment provides a monoclonal antibody that recognizes an anti-CLDN18.2 antibody, the light chain of the Fab segment of the monoclonal antibody is the amino acid sequence shown in SEQ ID NO.1, and the heavy chain of the Fab segment is SEQ ID NO.2 In the amino acid sequence shown, the light chain CDR1 is the amino acid sequence shown in SEQ ID NO.3, the light chain CDR2 is the amino acid sequence shown in SEQ ID NO.4, and the light chain CDR3 is the amino acid sequence shown in SEQ ID NO.5 , the heavy chain CDR1 is the amino acid sequence shown in SEQ ID NO.6, the heavy chain CDR2 is the amino acid sequence shown in SEQ ID NO.7, and the heavy chain CDR3 is the amino acid sequence shown in SEQ ID NO.8.

[0060] The preparation method of the monoclonal antibody comprises the following steps:

[0061] (1) Animal immunity

[0062] 6-week-old BALB / c female mice were used. The antigen was NA1-S (CN111978402A disclosed) digested by papain and the Fc segment was cut off...

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Abstract

The invention relates to a monoclonal antibody for identifying an anti-CLDN18.2 antibody and a preparation method and application thereof. A light chain CDR1 of the monoclonal antibody comprises an amino acid sequence shown in SEQ ID NO. 3; a light chain CDR2 comprises an amino acid sequence shown in SEQ ID NO. 4; a light chain CDR3 comprises an amino acid sequence shown in SEQ ID NO. 5; a heavy chain CDR1 comprises an amino acid sequence shown in SEQ ID NO. 6; a heavy chain CDR2 comprises an amino acid sequence shown in SEQ ID NO. 7; and a heavy chain CDR3 comprises an amino acid sequence shown in SEQ ID NO. 8. The monoclonal antibody can specifically identify anti-CLDN18.2 antibody NA1-S, does not block the combination of the anti-CLDN18.2 antibody and CLDN18.2, and can be applied to monitoring and evaluating the metabolic process of the anti-CLDN18.2 antibody.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a monoclonal antibody for recognizing anti-CLDN18.2 antibody and its preparation method and application. Background technique [0002] In recent years, antibody drugs have been widely used in the clinical treatment of various diseases such as cancer, transplant rejection, infectious diseases, inflammatory diseases and autoimmune diseases due to their high specificity, safety and controllability. The drug metabolism of antibody drugs is an important detection index in preclinical and clinical trial research. [0003] Claudin 18 is a tight junction protein. There are two spliced ​​isoforms in the human body, Claudin 18.1 and Claudin 18.2. Claudin 18.1 is mainly expressed in the lung, and Claudin 18.2 (CLDN18.2) is mainly expressed in the differentiated gastric mucosa. In epithelial cells, it is highly tissue specific. In addition, studies have shown that CLDN18.2 is highly expressed in...

Claims

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Application Information

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IPC IPC(8): C07K16/42C07K1/22C12N15/13C12N5/20G01N33/68G01N33/577G01N33/543C12R1/91
CPCC07K16/4258C07K2317/33C07K2317/51C07K2317/515C07K2317/565C07K2317/76G01N33/54306G01N33/577G01N33/686
Inventor 杨卫丽甄滢滢王涛袁雨许俊彦黄应峰邵喆
Owner DRAGONBOAT BIOPHARMACEUTICAL (SHANGHAI) CO LTD
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