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Micro-fluidic chip for separating and enriching exosomes and manufacturing method of micro-fluidic chip

A microfluidic chip, separation and enrichment technology, applied in laboratory containers, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of large consumption of sample reagents, long production time, low efficiency, etc. , to achieve the effect of reducing production cost and production time, low production cost and short capture time

Active Publication Date: 2021-06-18
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the present invention provides a microfluidic chip for separating and enriching exosomes and its manufacturing method, which overcomes the low efficiency of the traditional immunoaffinity method, large consumption of sample reagents, and long production time , high cost and other disadvantages

Method used

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  • Micro-fluidic chip for separating and enriching exosomes and manufacturing method of micro-fluidic chip
  • Micro-fluidic chip for separating and enriching exosomes and manufacturing method of micro-fluidic chip
  • Micro-fluidic chip for separating and enriching exosomes and manufacturing method of micro-fluidic chip

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Effect test

Embodiment 1

[0043] A microfluidic chip for separating and enriching exosomes, comprising a substrate with a three-dimensional porous structure bonded to a glass slide substrate, the substrate having a three-dimensional porous structure including microchannels arranged on the substrate, The microchannel is sequentially composed of a sample injection hole, a narrow channel one, a wide channel, a narrow channel two, and a sample outlet, and four three-dimensional porous regions with the same interval are arranged in the wide channel;

[0044] The pore area on the surface of the three-dimensional porous zone accounts for 21.6% of the total area of ​​the three-dimensional porous zone, and the average pore diameter on the surface of the three-dimensional porous zone is 2.94 μm; the pore area of ​​the cross-section of the three-dimensional porous zone accounts for 31.9% of the total area of ​​the three-dimensional porous zone. %, the average pore diameter of the cross-section of the three-dimensi...

Embodiment 2

[0058] A microfluidic chip for separating and enriching exosomes, comprising a substrate with a three-dimensional porous structure bonded to a glass slide substrate, the substrate having a three-dimensional porous structure including microchannels arranged on the substrate, The microchannel is sequentially composed of a sample injection hole, a narrow channel one, a wide channel, a narrow channel two, and a sample outlet, and four three-dimensional porous regions with the same interval are arranged in the wide channel;

[0059] The pore area on the surface of the three-dimensional porous zone accounts for 21.6% of the total area of ​​the three-dimensional porous zone, and the average pore diameter on the surface of the three-dimensional porous zone is 2.94 μm; the pore area of ​​the cross-section of the three-dimensional porous zone accounts for 31.9% of the total area of ​​the three-dimensional porous zone. %, the average pore diameter of the cross-section of the three-dimensi...

Embodiment 3

[0072] A microfluidic chip for separating and enriching exosomes, comprising a substrate with a three-dimensional porous structure bonded to a glass slide substrate, the substrate having a three-dimensional porous structure including microchannels arranged on the substrate, The microchannel is sequentially composed of a sample injection hole, a narrow channel one, a wide channel, a narrow channel two, and a sample outlet, and four three-dimensional porous regions with the same interval are arranged in the wide channel;

[0073]The pore area on the surface of the three-dimensional porous zone accounts for 21.6% of the total area of ​​the three-dimensional porous zone, and the average pore diameter on the surface of the three-dimensional porous zone is 2.94 μm; the pore area of ​​the cross-section of the three-dimensional porous zone accounts for 31.9% of the total area of ​​the three-dimensional porous zone. %, the average pore diameter of the cross-section of the three-dimensio...

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Abstract

The invention discloses a micro-fluidic chip for separating and enriching exosomes and a manufacturing method of the micro-fluidic chip. A soluble material is ground to a micron level, the soluble material is added into a plastic material as a pore-forming agent and then smeared at a wide channel of a micro-channel, and a three-dimensional porous area is obtained by washing off the pore-forming agent. The three-dimensional porous area has a unique three-dimensional porous structure, so that the capture efficiency of the separated and enriched exosomes is improved, the capture time of the required exosomes is short, the consumption of a sample reagent is small, and the manufacturing cost is low. The high-quality exosomes can be rapidly separated from serum and cell supernate, the manufacturing cost and the manufacturing time are greatly reduced, batch preparation can be achieved, miniaturization is achieved, and the method is suitable for clinically separating and enriching the exosomes on a large scale.

Description

[0001] field of invention [0002] The invention belongs to the field of biotechnology, and in particular relates to a microfluidic chip for separating and enriching exosomes and a manufacturing method thereof. Background technique [0003] Exosomes (Exosomes) are nanoparticles (30-150nm) wrapped in lipid membranes, which are derived from endocytosis. A type of outer vesicle. Exosomes carry and transmit important biological signals, which are closely related to biological processes such as cell communication, immune response regulation, tumorigenesis and metastasis, and affect the physiological and pathological state of the body. At present, exosomes have attracted the attention of researchers in the field of liquid biopsy, and it has been considered as an ideal biomarker for early diagnosis of tumors and prediction of tumor prognosis. [0004] However, due to the small size of exosomes (30-150nm), they are usually mixed with body fluids, which greatly increases the difficul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/00
CPCB01L3/502753B01L3/502707C12M47/04B01L2300/12B01L2200/10B01L2300/0874
Inventor 黄晓文陈嘉词张太毅李静王磊
Owner QILU UNIV OF TECH
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