The method of using lactic acid bacteria to improve the health function of cabbage
A technology of snail vegetables and lactic acid bacteria, which is applied in the direction of microorganism-based methods, lactobacilli, and the functions of food ingredients, can solve problems such as unseen technical reports, and achieve the effects of avoiding side effects, rich products, and clear fermentation conditions
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[0057] Example 1: Preparation of direct-throwing fermented inoculum and spinach kimchi
[0058] (1), in the MRS liquid medium of Lactobacillus plantarum ZJ316 of one inoculation loop to 10mL, activate for 36 hours at 37 DEG C; then successively pass 2 times according to the above-mentioned method, then connect to the inoculum according to 3% (volume %) In a 500 mL large bottle of MRS liquid medium, after culturing at 37 °C for 24 h, the fermentation broth was centrifuged (4 °C, 10000 × g, 20 min), the supernatant was removed, the bacterial pellet was taken, and the bacteria were washed three times with 0.85% saline (4 °C, 8000×g, 10min) to obtain bacterial paste.
[0059] (2), the formula of protective agent is: 10% skim milk powder, 1.5% trehalose, 1% glycerol, 3.5% sorbitol, and the balance is water; 3.5g of sorbitol, dilute to 100ml with water to obtain a protective agent.
[0060] Mix the protective agent and the bacterial slurry in a ratio of 1:3 (w / v, i.e. 1g / 3ml) of b...
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