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Method and system for screening siRNA sequences to reduce off-target effect

An off-target effect and candidate sequence technology, applied in bioinformatics, for the analysis of two-dimensional or three-dimensional molecular structure, informatics, etc., can solve the problems of low silencing efficiency and high off-target effect, to improve the breadth and reduce the possibility Effect

Pending Publication Date: 2021-06-11
NEOCURA BIO-MEDICAL TECH CO LTD
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Problems solved by technology

[0003] The present invention provides a method and system for screening siRNA sequences to reduce off-target effects, which are used to solve the problems of low silencing efficiency and high off-target effects in the prior art, by performing different dimensions of information on each candidate siRNA sequence Marking, considering whether it contains SNP sites, whether it can correctly identify the positive and negative strands, whether it can avoid autoimmune reactions, and whether it has sequence specificity, screen the original candidate siRNA sequence to obtain a series of silencing efficiency improvement and off-target siRNA candidate sequences with reduced effect

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  • Method and system for screening siRNA sequences to reduce off-target effect
  • Method and system for screening siRNA sequences to reduce off-target effect

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Embodiment Construction

[0056] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0057] A method for screening siRNA sequences proposed in the embodiments of the present invention to reduce off-target effects, such as figure 1 and figure 2 As shown, the method includes:

[0058] Step S1: Integrate the siRNA candidate sequence data, and mark the genomic interval of the target region of the siRNA sequence with or without common SNPs with a minor allele frequency of 1% or more;

[0059] Step S2: Analyze the characteristics of the positive and negative strand sequences of the siRNA, and mark whether the positive and negative strand sequences of the siRNA can correctly identify the positive and negative strands;

[0060] Step S3: analyzing the neg...

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Abstract

The invention provides a method and system for screening siRNA sequences to reduce off-target effect. The method comprises the following steps: S1, integrating siRNA candidate sequence data, and marking whether common SNP exists in a siRNA sequence target region genome interval or not; S2, analyzing the characteristics of the siRNA positive and negative chain sequences, and marking whether the positive and negative chains can be correctly recognized or not on the siRNA positive and negative chain sequences; S3, analyzing the negative strand characteristics of the siRNA sequence, and labeling whether the negative strand of the siRNA sequence can trigger an autoimmune reaction or not; S4, analyzing the specificity of the siRNA positive and negative chain sequences, and carrying out siRNA sequence specificity labeling on the siRNA positive and negative chain sequences; and S5, synthesizing all the labeling results, and screening the candidate siRNA sequences. The system comprises unit modules corresponding to the steps of the method.

Description

technical field [0001] The invention relates to the field of disease treatment of small molecule interfering RNA (siRNA) technology, in particular to a method and system for screening siRNA sequences to reduce off-target effects. Background technique [0002] In recent years, the continuous deepening of RNAi research has opened up a new path for gene therapy of diseases. Small interfering RNA is precisely targeted to the mRNA of the target gene through the principle of complementarity to achieve the effect of silencing the gene. Because of this clear and rapid gene post-transcriptional regulation ability, siRNA technology has become one of the most effective means to develop drugs. Compared with traditional small molecule and antibody drug treatments, siRNA molecule drugs have technical advantages such as long half-life, convenient administration, and high efficiency. However, with the continuous expansion of the field of RNAi therapy and the continuous in-depth research o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B15/30
CPCG16B15/30
Inventor 万季沈一鸣刘鹏徐韵婉潘有东王弈宋麒
Owner NEOCURA BIO-MEDICAL TECH CO LTD
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