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Gastrointestinal flora determination method

A technology of gastrointestinal flora and measurement method, applied in the field of gastrointestinal flora measurement, can solve problems such as colony imbalance and aggravation of disease, and achieve scientific and reasonable results of the measurement method

Inactive Publication Date: 2021-06-11
武汉普渡生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is a large number of complex bacterial groups in the human intestine, which is also the largest and most complex microbial community in the human body. Under normal circumstances, the intestinal flora maintains a balance with the human body and the external environment, which plays an important role in the health of the human body. At the same time, many diseases can also cause the imbalance of bacterial colonies, thus aggravating the disease. The analysis of intestinal bacteria is a very complicated work. With the development of detection technology, the understanding of intestinal bacteria is also constantly To advance, the current determination of gastrointestinal flora usually requires very skilled technicians to observe and interpret the test results, and a simple and accurate method is needed to determine the number of microorganisms in liquid samples; for this reason, we propose a gastrointestinal Method for the determination of bacteria flora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for measuring gastrointestinal flora, comprising the following steps:

[0021] S1: Weigh 30g of feces sample and add it to the high-speed mashing cup. Thaw the frozen sample at 1°C (15h). To melt, add 300ml of phosphate buffer solution and stir for 3 minutes to make a 1:10 sample solution;

[0022] S2: The liquid in the mashing cup should completely cover the blade. The dilution factor is based on the contamination of the sample by the coliform group. Use the diluent to make a series of ten-fold incremental sample dilutions, and shake the sample evenly;

[0023] S3: Select appropriate sample dilutions of three serial dilutions, inoculate 3 tubes of LST for each dilution, inoculate 1ml in each tube, and make the tip of the pipette stay on the wall of the test tube at a certain angle for 2 seconds, from the preparation of the sample Homogenize the solution until the dilution is complete;

[0024] S4: Inhale three tubes of lauryl sulfate tryptin (LST) broth into ...

Embodiment 2

[0033] A method for measuring gastrointestinal flora, comprising the following steps:

[0034] S1: Weigh 35g of feces sample and add it to the high-speed mashing cup. Thaw the frozen sample at 2°C (16h). To thaw, add 350ml of phosphate buffer solution and stir for 4 minutes to make a 1:10 sample solution;

[0035] S2: The liquid in the mashing cup should completely cover the blade. The dilution factor is based on the contamination of the sample by the coliform group. Use the diluent to make a series of ten-fold incremental sample dilutions, and shake the sample evenly;

[0036] S3: Select appropriate sample dilutions of three serial dilutions, inoculate 3 tubes of LST for each dilution, and inoculate 1ml in each tube. At a certain angle, make the tip of the pipette stay on the wall of the test tube for 2.5 seconds. Homogenize the sample until the dilution is complete;

[0037] S4: Inhale three tubes of lauryl sulfate tryptin (LST) broth into the sample test tube with a straw,...

Embodiment 3

[0046] A method for measuring gastrointestinal flora, comprising the following steps:

[0047] S1: Weigh 40g of feces samples into the high-speed mashing cup, thaw the frozen samples at 3°C ​​(14h), add 400ml of phosphate buffer saline, and stir for 5 minutes to make a 1:10 sample solution;

[0048] S2: The liquid in the mashing cup should completely cover the blade. The dilution factor is based on the contamination of the sample by the coliform group. Use the diluent to make a series of ten-fold incremental sample dilutions, and shake the sample evenly;

[0049] S3: Select appropriate sample dilutions of three serial dilutions, inoculate 3 tubes of LST for each dilution, inoculate 1ml in each tube, and make the tip of the pipette stay on the wall of the test tube at a certain angle for 3 seconds, from the preparation of the sample Homogenize the solution until the dilution is complete;

[0050] S4: Inhale three tubes of lauryl sulfate tryptin (LST) broth into the sample test...

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Abstract

The invention discloses a gastrointestinal flora determination method, which relates to the technical field of microbiological detection, and comprises the following steps: preparing a sample solution, shaking, diluting, culturing, carrying out a confirmation test, carrying out water bath, carrying out gas production contrast, carrying out taxonomic analysis and sequencing. The measuring method is more scientific and reasonable, the number of microorganisms in a liquid sample and the intestinal flora structure can be accurately measured through the number of gas production pipes, an intestinal flora sequencing report can be obtained, the human health development trend can be scientifically predicted, people health management can be guided, deviation of bacterial microbiota can be quantitatively measured, and treatment that can make up or at least partially address a gastrointestinal dysbiosis or extent thereof in an individual.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a method for measuring gastrointestinal flora. Background technique [0002] The gastrointestinal tract is a continuous series of organs beginning at the mouth and ending at the anus, and throughout its length the gastrointestinal tract is colonized with microorganisms of many different species, collectively, the microbial content of the gastrointestinal tract is the microbiota of the gastrointestinal tract , the relative amount of constituent microorganisms or groups thereof can be considered as the distribution of microbiota, which thus yields information about the microbial diversity in the gastrointestinal tract, as well as providing information about the relative amounts of microorganisms or groups of them present. information; [0003] There is a large number of complex bacterial groups in the human intestine, which is also the largest and most complex micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/04
CPCC12Q1/04C12Q1/06
Inventor 张小燕
Owner 武汉普渡生物医药有限公司
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