Method for preparing cell wall polysaccharide by utilizing ganoderma lucidum spore powder raffinate and product and application thereof

A technology of Ganoderma lucidum spore powder and broken Ganoderma lucidum spore powder, which is applied in the field of preparing cell wall polysaccharides from the extract residue of Ganoderma lucidum spore powder, can solve the problems of polysaccharide raw material waste, etc., and achieve the goal of improving utilization rate, improving economic benefits, and good economic benefits Effect

Active Publication Date: 2021-06-04
SHANGHAI BAIXIN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the main functional components of Ganoderma lucidum spore oil products are triterpenes, and the content of polysaccharides that can enhance immunity is very small, and after the spore powder is extracted and oiled, the extracted residue of Ganoderma lucidum spore powder still contains a large amount of polysaccharides. If the ganoderma lucidum spore powder extract residue is not recycled, a large amount of polysaccharide raw materials will be wasted

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  • Method for preparing cell wall polysaccharide by utilizing ganoderma lucidum spore powder raffinate and product and application thereof
  • Method for preparing cell wall polysaccharide by utilizing ganoderma lucidum spore powder raffinate and product and application thereof

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Embodiment 1

[0055] This embodiment provides a method for preparing cell wall polysaccharides from Ganoderma lucidum spore powder raffinate, said method comprising the following steps:

[0056] (1) In the BFM100B ultrafine powder vibrating mill, the extract residue of Ganoderma lucidum spore powder was subjected to wall-breaking treatment at -5°C, and the time of wall-breaking treatment was 20 minutes to obtain the extract residue of broken wall Ganoderma lucidum spore powder;

[0057] (2) Use ultra-pure water to carry out extraction treatment to step (1) obtained broken ganoderma lucidum spore powder raffinate under stirring condition at 90 ℃, the liquid-to-material ratio of extraction treatment is 20:1, and the time of extraction treatment is After 200min, the extract was obtained after solid-liquid separation;

[0058] (3) The extract obtained in step (2) was allowed to stand at 20° C. for 10 h, and then subjected to solid-liquid separation to obtain a supernatant;

[0059] (4) carry o...

Embodiment 2

[0062] This embodiment provides a method for preparing cell wall polysaccharides from Ganoderma lucidum spore powder raffinate, said method comprising the following steps:

[0063] (1) In the BFM100B ultrafine powder vibrating mill, the extract residue of Ganoderma lucidum spore powder was subjected to wall-breaking treatment at 0° C., and the time of wall-breaking treatment was 30 minutes to obtain the extract residue of broken wall Ganoderma lucidum spore powder;

[0064] (2) Use purified water to extract the extract of the broken Ganoderma lucidum spore powder obtained in step (1) at 60°C under stirring conditions, the liquid-to-solid ratio of the extraction process is 25:1, and the extraction process takes 150 minutes , to obtain the extract after solid-liquid separation;

[0065] (3) The extract obtained in step (2) was left to stand at 25° C. for 9 hours, and then subjected to solid-liquid separation to obtain a supernatant;

[0066] (4) performing butterfly centrifugat...

Embodiment 3

[0069] This embodiment provides a method for preparing cell wall polysaccharides from Ganoderma lucidum spore powder raffinate, said method comprising the following steps:

[0070] (1) In the BFM100B superfine powder vibrating mill, the extract residue of Ganoderma lucidum spore powder was subjected to wall-breaking treatment at 5° C., and the time of wall-breaking treatment was 40 minutes to obtain the extract residue of Ganoderma lucidum spore powder;

[0071] (2) Use purified water to extract the ganoderma lucidum spore powder extract obtained in step (1) under stirring conditions at 95°C, the liquid-to-solid ratio of the extraction treatment is 15:1, and the extraction treatment time is 250min , to obtain the extract after solid-liquid separation;

[0072] (3) The extract obtained in step (2) was allowed to stand at 25° C. for 11 h, and then subjected to solid-liquid separation to obtain a supernatant;

[0073] (4) carry out butterfly centrifugation and tubular centrifuga...

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Abstract

The invention provides a method for preparing cell wall polysaccharide by utilizing ganoderma lucidum spore powder raffinate and a product and application thereof, and the method comprises the following steps: carrying out wall-breaking treatment on the ganoderma lucidum spore powder raffinate to obtain wall-broken ganoderma lucidum spore powder raffinate; carrying out extraction treatment on the wall-broken ganoderma lucidum spore powder raffinate, and carrying out solid-liquid separation to obtain an extracting solution; standing the extracting solution, and then carrying out solid-liquid separation to obtain supernate; sequentially carrying out primary centrifugation and secondary centrifugation on the supernate to obtain centrifugate; and concentrating the centrifugate, and drying to obtain the cell wall polysaccharide. The ganoderma lucidum spore powder raffinate is recycled, the utilization rate of the ganoderma lucidum spore powder raw material is increased, and the prepared polysaccharide is cell wall polysaccharide with good moisturizing activity, can be used as a high-quality raw material of various chemical products and has good economic benefits.

Description

technical field [0001] The invention belongs to the technical field of edible fungus extraction, and relates to a method for preparing polysaccharides and its products and applications, in particular to a method for preparing cell wall polysaccharides from Ganoderma lucidum spore powder extract and its products and applications. Background technique [0002] Ganoderma lucidum is the treasure of traditional Chinese medicine. It has the function of nourishing and strengthening. The polysaccharides in the spores of Ganoderma lucidum and the polysaccharides in the fruiting body of Ganoderma lucidum are different in structure, and their efficacy and effect are also different. Ganoderma lucidum spores are oval germ cells produced during the growth and maturity stage of Ganoderma lucidum. They contain all the genetically active substances of Ganoderma lucidum. The active ingredients are mainly polysaccharides, triterpenoids and factors related to life metabolism. Modern research sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/074A61K31/715A61K39/00A61K8/9728A61K8/73A61Q19/00
CPCC08B37/0003A61K36/074A61K31/715A61K39/00A61K8/9728A61K8/73A61Q19/00A61K2800/805
Inventor 唐庆九王晨光周靖王金艳刘艳芳
Owner SHANGHAI BAIXIN BIO TECH
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