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Liquid crystal biosensor capable of simultaneously detecting multiple cancer markers and preparation and detection method of liquid crystal biosensor

A technology of cancer markers and biosensors, applied in the field of analysis and detection, can solve problems such as difficult coding and restrictions on the practical application of liquid crystal biosensors

Pending Publication Date: 2021-05-18
SHANDONG ANALYSIS & TEST CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the liquid crystal optical signal has only two forms of bright and dark, it is difficult to code, and the liquid crystal sensor that can simultaneously detect multiple substances has not yet been realized, which seriously restricts the practical application of liquid crystal biosensors in clinical cancer diagnosis.

Method used

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  • Liquid crystal biosensor capable of simultaneously detecting multiple cancer markers and preparation and detection method of liquid crystal biosensor
  • Liquid crystal biosensor capable of simultaneously detecting multiple cancer markers and preparation and detection method of liquid crystal biosensor
  • Liquid crystal biosensor capable of simultaneously detecting multiple cancer markers and preparation and detection method of liquid crystal biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Preparation of liquid crystal biosensor

[0059] A method for preparing a liquid crystal biosensor for simultaneously detecting multiple cancer markers, the steps are as follows:

[0060] (1) Preparation of glass substrate: Soak the glass slide in 60mL of the washing solution prepared by the volume ratio of 98% concentrated sulfuric acid and 30% hydrogen peroxide at a volume ratio of 7:3. soak for 30 minutes, then wash three times with water, absolute ethanol, and methanol respectively, blow dry with nitrogen, dry at 110°C for 12 hours, place in DMOAP solution with a mass concentration of 0.5% at room temperature for 15 minutes, and wash with three times distilled water for 5 minutes. Second, blow dry with nitrogen, solidify at 105°C for 3 hours, and obtain a glass substrate after cooling;

[0061] (2) Preparation of multi-channel sample tank: 3D printing multi-channel sample tank, multi-channel sample tank includes individual channels and connecting chan...

Embodiment 2

[0066] Example 2: Method for Simultaneous Detection of Multiple Cancer Markers

[0067] A method for simultaneously detecting carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and alpha-fetoprotein (AFP), the steps are as follows:

[0068] S1. Preparation of capture magnetic bead probes: Mix 10 μL 0.1 mg / mL streptavidin-modified magnetic bead dispersion with 10 μL 0.4 μM biotin-modified CEA aptamer 1 solution, prostate-specific antigen Aptamer 1 solution and alpha-fetoprotein aptamer 1 solution were co-incubated at 37°C for 0.5 h, then the magnetic beads were separated from the solution using a commercially available magnetic separation rack, the solution was discarded, and the magnetic beads were washed with phosphate buffer solution 3 Once, three kinds of capture magnetic bead probes were prepared, and then the three kinds of capture magnetic bead probes (1 μg each) were dispersed into 30 μL phosphate buffer at the same time, and shaken to obtain a mixed disper...

Embodiment 3

[0080] Embodiment 3: Detect CEA, PSA and AFP of unknown concentration in blood

[0081] The preparation of capture magnetic bead probe and double-stranded signal probe is the same as that in Example 2.

[0082] The blood sample is tested immediately after being drawn, and the sample used in this embodiment is a blood sample of a healthy person.

[0083] Take 2 μL blood sample, dilute it 5 times with phosphate buffer, mix the dispersion with 10 μL 0.1 mg / mL capture magnetic bead probe, incubate at 37°C for 30 min, separate the magnetic beads with a magnetic frame, discard the solution, and use Wash 3 times with phosphate buffer, disperse the washed capture magnetic bead probes into 100 μL 10pM double-stranded signal probe mixed solution, incubate at 37°C for 30 min, separate the magnetic beads with a magnetic stand, and take the supernatant solution was added to the communication channel of the multi-channel sample tank of the liquid crystal biosensor prepared in Example 1, an...

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Abstract

The invention relates to a liquid crystal biosensor for simultaneously detecting multiple cancer markers and a preparation and detection method thereof. The liquid crystal biosensor provided by the invention comprises a multi-channel sample groove and a liquid crystal biosensing interface modified by different cancer marker coding DNA (Deoxyribose Nucleic Acid), the multi-channel sample groove comprises an independent channel and a communicating channel, and the communicating channel is located above the independent channel, so that the upper half parts of different independent channels are communicated with one another; and the liquid crystal biosensing interfaces modified by the different cancer marker coding DNA are constructed in different independent channels of the multi-channel sample groove. Different cancer markers are utilized to encode DNA to modify the liquid crystal biosensor, simultaneous detection and quantitative detection of various different cancer markers are realized, accurate detection of extreme samples is also realized, and the detection method has the advantages of small sample dosage, convenience in detection, rapidness, high efficiency, high sensitivity, low cost and no need of large instruments.

Description

technical field [0001] The invention relates to a liquid crystal biosensor for simultaneously detecting multiple cancer markers and a preparation and detection method thereof, belonging to the technical field of analysis and detection. Background technique [0002] With the continuous advancement of science and technology, the continuous improvement of living standards, and the continuous extension of human life expectancy, cancer has gradually become one of the diseases that threaten human life and health. The detection of cancer markers with high specificity and high sensitivity plays an important role in the early diagnosis, drug intervention and prognostic treatment of cancer. In practical clinical applications, it is often necessary to detect multiple markers to improve the accuracy of detection and exclude false positive results. Therefore, the development of multiple detection technology can reduce sample consumption, save operating costs, shorten detection time, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N21/84
CPCG01N33/574G01N33/57473G01N33/57476G01N21/84
Inventor 胡琼政亓鲁滨刘淑雅于丽
Owner SHANDONG ANALYSIS & TEST CENT
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