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Application of CH223191 in inhibition of tumor cell migration

A technology of CH223191, 1. CH223191, applied in the application of inhibiting the migration of glioblastoma cells, in the field of cancer treatment, can solve the problems of low long-term survival rate and poor prognosis

Inactive Publication Date: 2021-05-18
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Comprehensive treatment of glioblastoma includes surgery, radiotherapy, systemic therapy (chemotherapy, targeted therapy) and supportive care, etc., but the overall prognosis is still poor, long-term low survival rate

Method used

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  • Application of CH223191 in inhibition of tumor cell migration
  • Application of CH223191 in inhibition of tumor cell migration
  • Application of CH223191 in inhibition of tumor cell migration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053]Example 1CH223191 Inquiry of toxicity effect on glue skeletarns

[0054]U87 cells in 1 × 105The concentration of / ml was inoculated into a 96-well plate, 100 ml of cell suspension per well. Overnight culture waiting for cellular wall. 96-well plates were taken, and the original medium was cleaned, washed once with phosphate buffer (PBS). The experiment is set up background control group, solvent control group and pharmaceutical treatment group. The corresponding low serum medium (serum concentration of 1%, context-free concentration), solvent-containing dimethyl sulfoxide (DMSO) or different concentrations of low serum medium, and maintains the volume of the solvent 0.1%. The 96-well plate after the dosing was placed in the incubator to continue to cultivate 24h or 48 h. After arriving at the endpoint of the culture, 10 μl of CCK8 reagent was added to each well, and the hole plate was added to the incubator in the incubator for 1 h, and the absorbance value was read with the enz...

Embodiment 2

[0056]Example 2. Inhibition of Migration of U87 Cells in CH223191

[0057]It is used to evaluate the migration of cells by scratch experiments. The scratches are positioned with a marker stroke at the bottom of the 6-well plate. Cell in 4 × 105The density of / ml inoculated 2ml in 6-well plates, overnight culture waiting for cellular wall. After the cells are completely covered by bottom of the well plate, the cell monolayer is scratched with a 1 mL pipette tip to form a scratch. Select scratches to the junction of the Mark's stroke as a photographic location. The removal of floating cells was washed with phosphate buffer, and then a low serum medium containing solvent DMSO or CH223191 was added. The image is collected under the inverted microscope 0-48h after scratching. A total of three independent repetition experiments were performed. Each concentration group in each independent experiment has three repeating holes, and each hole contains three scratches, and 12 images are taken an...

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Abstract

The invention relates to application of CH223191 in inhibition of cell migration, in particular to application of CH223191 or a pharmaceutical composition containing the CH223191 in preparation of a medicine for inhibiting glioma cell migration. It is found for the first time that the CH223191 with a specific concentration can inhibit migration of a glioblastoma cell line U87, and a new choice is provided for treatment of glioblastoma.

Description

Technical field[0001]The present invention relates to the field of molecular biology, and more particularly, the present invention relates to cancer treatment related technologies. The present invention mainly includes the application of CH223191 in inhibiting tumor cell migration, and is particularly disclosed in the migration of CH223191 in inhibiting glioblasty cell tumor cells.Background technique[0002]In all cancer-related cases, brain and central nervous system tumors are the third cause of cancer-related death. In the brain tumor, the amazing rate is high, accounting for 15.1% of the primary central nervous system tumor, accounting for 46.1% of the malignant tumors of the primary central nervous system. Among them, glue skeletarns are most common, belonging to a very high invasive primary glioma, and is also a magic tumor (WHO) tumor malignant classification in the classification of No. IV.[0003]Comprehensive treatment of gliobate skeletarns includes surgery, radiotherapy, sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/415A61P35/00A61P35/04
CPCA61K31/415A61P35/00A61P35/04
Inventor 赵斌刘奕耘谢群慧徐丽陈旸升
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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