Large-volume biological cell sap concentrating and washing system and method thereof

Abstract

The invention provides a large-volume biological cell sap concentrating and washing system and a method thereof. The large-volume biological cell sap concentrating and washing system comprises a centrifugal assembly and a concentrating and washing assembly, wherein the centrifugal assembly comprises a centrifugal machine, the concentrating and washing assembly comprises a centrifugal cup, the centrifugal cup is installed on the centrifugal machine, and the centrifugal machine drives the centrifugal cup to rotate at a high speed; the centrifugal cup comprises a static part and a rotating part, the static part and the rotating part are in rotary sealing fit, the rotating part comprises a separation cavity, the separation cavity is a cavity formed by a cup body and an inner core, and when the centrifugal cup rotates at a high speed, cells and liquid in a cell sap sample are separated in the separation cavity. According to the large-volume biological cell sap concentrating and washing system and method, the movement path of the cell sap is long, and centrifugal efficiency is high; meanwhile, the inner core is additionally arranged in the separation cup, so the dynamic volume of the separation cup can be adjusted, the minimum centrifugal force is ensured, the separation effect is further ensured, and the cell recovery rate is improved.

Description

technical field [0001] The invention belongs to the field of automatic processing and separation of biological cells, and in particular relates to a large-volume biological cell liquid concentration washing system and a method thereof. Background technique [0002] Cell therapy refers to a treatment method that uses the patient's own (or allogeneic) adult cells (or stem cells) to repair tissues and organs. Widely used in bone marrow transplantation, advanced liver cirrhosis, femoral head necrosis, malignant tumors, myocardial infarction and other diseases. Currently, therapeutic cells are generally derived from umbilical cord blood, bone marrow, peripheral blood and fat. Because their content is very little, it is generally necessary to carry out cell culture to make its number amplified to a certain amount (for example, the number of cells obtained is 1×10 9 , which needs to be increased by a factor of 10 to reach 1×10 10 indivual). During the cell culture process, it i...

AI Technical Summary

Problems solved by technology

[0007] In summary, the cell recovery rate of the current processing equipment is not high, and the efficiency is low when concentrated washing with a large amount of cell fluid

In addition to the above methods, when the amount of cell fluid to be processed is large, the operator will also use manual processing, but the manual processing is cumbersome, time-consuming and labor-intensive, the result consistency is not good, and the recovery efficiency varies greatly. with the possibility of contamination

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