Molecular markers for auxiliary diagnosis of viral meningitis and their applications and kits
A technology of viral meningitis and molecular markers, applied in biochemical equipment and methods, microbe determination/testing, DNA/RNA fragments, etc., can solve problems such as confusion of other diseases, low positive detection rate, and difficulty in finding lesions , to achieve the effect of improving early diagnosis rate, reducing mortality rate and high diagnostic value
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Embodiment 1
[0079] Example 1: density and boxplot analysis results
[0080] By extracting small RNAs from the cerebrospinal fluid of patients with viral meningitis infection and healthy adults, miRNA identification was performed, and the bioinformatics analysis statistics were density and boxplot analysis graphs ( figure 2 ), it was found that there was no difference in the overall number of miRNAs in the diseased brain group and the control group.
Embodiment 2
[0081] Embodiment 2: density and boxplot analysis graph PCA and volcano plot analysis result graph
[0082] By extracting small RNAs from the cerebrospinal fluid of patients with viral meningitis infection, patients with non-viral infection, and healthy adults, miRNA identification was performed, and the bioinformatics analysis and statistics were PCA and volcano plot analysis graphs (such as image 3 ), it was found that the overall number of miRNAs in the diseased brain group and the control group was almost the same.
Embodiment 3
[0083] Example 3 Heatmap
[0084] By extracting small RNAs from the cerebrospinal fluid of patients with viral meningitis infection and healthy adults, miRNA identification was performed, and the differential expression of differentially expressed genes in different samples was visualized by Heatmap (such as Figure 4 ).
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