Nucleotide compositions for inhibiting non-target regions in biological DNA samples and their applications

Abstract

The invention relates to a nucleotide composition for inhibiting a non-target region in a biological DNA sample and its application. The nucleotide chain composition of the present invention specifically binds to a non-target region so that the non-target region is not amplified non-specifically. increase, recognition, capture, etc.; or, the nucleotide chain composition specifically binds to the target region, further causing the non-target region to be degraded or eliminated, so that the non-target region is not non-specifically amplified, recognized, captured, etc. . The nucleotide chain composition for inhibiting non-target regions in biological DNA samples disclosed by the present invention has the advantages of fast hybridization speed, high specificity, single base difference recognition, etc., and is especially suitable for the removal of heterogeneous chromatin and highly homologous region information Molecular detection fields such as detection and identification of true and false genetic information.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a nucleotide composition for inhibiting non-target regions in biological DNA samples and an application thereof. Background technique [0002] With the development of science and technology, researchers are increasingly aware that biological DNA samples are usually a complex sample, for example, saliva samples, we all know that there are many bacteria in the mouth, as well as food residues, from When extracting DNA from saliva samples, about 10% of the DNA is not of human origin; for another example, blood samples were considered sterile under natural conditions, and the extracted DNA was naturally of human origin, but , with the development of detection technology and the deepening of analytical understanding, it is now found that some of the free DNA in human blood samples comes from bacteria or other microorganisms; even pure human genomic DNA, there will be...

AI Technical Summary

Problems solved by technology

[0004] With the increasing refinement and precision of technology and analysis methods, people will inevitably require a way to identify the target area more finely, so as to reduce the roughness of obtaining target information, reduce the cost of obtaining target information, and even require Target information can be more accurately identified at the setting stage. For example, in the field of cancer target drug therapy, there is a one-to-one correspondence between drugs and target information. Even if other irrelevant DNA information is obtained, it will not allow doctors to make more decisions. Patients cannot benefit more from drug decision-making, because the types and scope of application of drugs are very clear and limited, which requires detection technology to tend to obtain more specific and clear data rather than broader data. It can be seen that, On the same drug target, for example, at the position of EGFR 2390, in addition to the reference sequence information G, there can also be three variations of A / T / C, and these three variations, only G>C and G>A The change has the meaning of guiding treatment, and the information of G>T has no medical value at present. However, some detection methods for this position, such as the Taqman probe method, can only identify the mutation of this G, but cannot Confirm which one is G>C, G>A and G>T, which will easily cause false positive results

Images