Maize 100-kernel weight major QTL site qkw4a and its candidate genes and applications

A technology of candidate gene and hundred-seed weight, applied in application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2022-05-03
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there are no related reports on the fine mapping of KW main QTL loci (qKW4a) and the confirmation of candidate genes based on map-based cloning

Method used

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  • Maize 100-kernel weight major QTL site qkw4a and its candidate genes and applications
  • Maize 100-kernel weight major QTL site qkw4a and its candidate genes and applications
  • Maize 100-kernel weight major QTL site qkw4a and its candidate genes and applications

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Embodiment 1

[0018] 1. Fine mapping of the main KW QTL locus qKW4a in maize

[0019] The present invention is implemented based on the preliminary QTL mapping results of KW in maize (identification of 100-kernel weight QTL in maize based on Ye 478 introduction line, Zhan Jingjing et al., 2015), that is, the locus qKW4a controlling KW is located at the main point between bnlg1784-umc1194 in maize 4.07 bin Effective QTL loci, its phenotype contribution rate is 17.81% ( figure 1 A).

[0020] On the basis of previous research (identification of 100-kernel weight QTL in maize based on Ye 478 introduction line, Zhan Jingjing et al., 2015), the present invention utilizes 210 BC 1 The population (derived from the hybridization of Y478 and SL41, wherein Y478 is an excellent inbred line of maize in my country, Ye 478, and SL41 is a chromosome single-segment substitution line introduced into QB80 with Y478 as the genetic background), was used to detect the developed markers of each individual plant,...

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Abstract

The invention discloses the main effect QTL site qKW4a of 100-kernel weight in maize, its candidate gene and application, and belongs to the field of crop genetics and breeding. The present invention uses SL41 and Ye478 as parents to construct a genetic segregation population, and through map-based cloning, the main QTL site qKW4a for 100-kernel weight in maize is finely positioned between the chr4 markers kw4–kw10 of the B73 reference genome, and based on the fine-mapping segment Gene expression patterns, genomic and cDNA sequence differences between the parents (SL41 and Ye478), and enzymatic activities and cytological features during grain starch formation identified ZmSSV, a candidate gene for qKW4a. The candidate gene ZmSSV isolated and cloned by the map position cloning method of the present invention is related to maize KW (kernel weight), and will be effectively used for molecular breeding and genetic improvement of maize KW.

Description

technical field [0001] The invention relates to the field of crop genetics and breeding, in particular to the main QTL site qKW4a for 100-kernel weight in maize, its candidate genes and applications thereof. Background technique [0002] Corn hundred-kernel weight (100-kernel weight, 100-KW; or kernel weight, KW) is the main determinant of single ear yield. Therefore, the mapping of grain weight QTL (or map-based cloning) and the cloning of its candidate genes will contribute to the improvement of grain yield and food security in my country. Maize KW is a quantitative trait controlled by multiple genes, and map-based cloning is recognized as an effective method for analyzing the genetic mechanism of quantitative traits and isolating candidate genes. And it has been shown that maize KW can be controlled by a few major QTLs. [0003] Maize KW is usually the accumulation of dry matter in the kernel, and it is mainly starch. The isolation and cloning of endosperm cell divisio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12Q1/6895
CPCC12N9/1051C12Q1/6895C12Y204/01011C12Q2600/13C12Q2600/158
Inventor 陶勇生段会军张大效马小林
Owner HEBEI AGRICULTURAL UNIV.
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