Reaction module and corresponding multiple and multi-channel real-time fluorescent nucleic acid detector

Abstract

The invention relates to a reaction module for a multiple and multi-channel real-time fluorescent nucleic acid detector. The reaction module is a compact overall structure and formed by a heating member assembly and an optical fiber pair; the heating member assembly includes a heating module, a cooling module and a temperature controlling panel; the heating module includes heating pieces used for inserting and heating reaction tubes; the cooling module includes a fan used for reducing temperatures; and the optical fiber pair includes incident optical fiber and emergent optical fiber in mutually parallel. A multiple and multi-channel real-time fluorescent nucleic acid detector adopting the reaction module is also related. The detector includes a light source excitation and fluorescence detection assembly, a motion assembly, a computer and an external power supply; and the heating pieces, the fan and the optical fiber pair are located in the space enclosed by shell plate of the reaction module. Therefore, requirements for detecting few samples while arriving can be met; and conditions can be created for the nucleic acid detector to enter primary inspection institutions and small and medium-sized hospitals and be used for bedside detection.

Description

Technical field[0001]The present invention relates to the field of nucleic acid amplification detection, and more particularly to a reaction module and a corresponding multiple multi-channel real-time fluorescent nucleic acid detector.Background technique[0002]The nucleic acid detector is an instrument for amplifying and detecting a nucleic acid by a PCR technology or a constant temperature amplification method. The real-time fluorescent nucleic acid detector is the accumulation amount of the fluorescent dye generated by the fluorescent dye or specific probe added to the reaction system. Instruments reflecting the amount of amplified product.[0003]When PCR amplification and constant temperature amplification, the real-time fluorescent nucleic acid detector needs to use a light source to illuminate the reaction liquid in the reaction tube, and then detect the fluorescent dye or fluorescent probes produced by the reaction liquid by the detector. The traditional real-time fluorescent n...

AI Technical Summary

Problems solved by technology

The more reaction wells the instrument has, the more suitable it is for batch sample detection. However, the more wells the instrument has, the more complex the structure of the instrument may be, and the volume, weight and cost of the instrument will also be lower. will increase, and for users who have a small sample size but need to be tested at any time, in order to reduce the start-up costs and depreciation costs of the instrument, it is often necessary to collect multiple samples and put them on the machine together, which will prolong the time for the test results to be given. , Delay the diagnosis of the disease, and even lead to the accelerated spread of infectious diseases among susceptible people, and if only a small number of samples are tested at a time, the hole position and power will be wasted, and the utilization efficiency and service life of the instrument will be reduced. An instrument that can be checked at any time

[0006] The current nucleic acid detectors and real-time fluorescent nucleic acid detectors have the advantages of high throughput and powerful functions, but they also have the disadvantages of being expensive, bulky, heavy, and limited in the number of detection channels, making it difficult to enter grassroots inspection institutions, small and medium-sized hospitals and for point-of-care testing

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