A kind of blood wood tissue culture method

A technology of tissue culture and bloodwood, which is applied in the field of plant biology, can solve the problems of limited number of ring branch propagation, difficulty in obtaining a large number of seedlings, and low natural seed setting rate, and achieve good market prospects, low investment, and strong resistance. Effect

Active Publication Date: 2022-06-07
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bloodwood can be propagated by sowing, but its natural seed setting rate is low, and the embryos are often underdeveloped, resulting in low germination rate
The number of ring branch propagation of pig blood wood is also very limited, so it is difficult to obtain a large number of seedlings through conventional propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Disinfection method of explants: take the blue-black fruit of Euryodendron excelsum H.T.Chang on the vigorously growing plant, soak in water and remove the pulp to obtain well-developed seeds, first soak in 75% ethanol aqueous solution 10 seconds, then disinfected with a mass fraction of 0.1% mercuric solution for 10 minutes, rinsed 5 times with sterile water, and inoculated into the seed germination medium, and the disinfection success rate was 95%. The culture temperature was 24°C, the light intensity was 1500lx, and the light was 16 hours / day. Seeds germinate after 30 days.

[0022] The seed germination medium consists of the following components per liter: 20 g of sucrose, 6 g of agar, and the balance is 1 / 2 MS medium, pH 5.8.

[0023] 2. Induction of adventitious buds: 40 days after seed germination, when the seedling height is 1 cm, cut the hypocotyl and inoculate it on the adventitious bud induction medium to induce adventitious buds. The culture temperature...

Embodiment 2

[0032] 1. Disinfection method of explants: take the blue-black fruit of Euryodendron excelsum H.T.Chang on the vigorously growing plant, soak in water and remove the pulp to obtain well-developed seeds, first soak in 75% ethanol aqueous solution 20 seconds, then disinfected with a mass fraction of 0.1% mercuric solution for 8 minutes, rinsed 5 times with sterile water, and inoculated into the seed germination medium, and the disinfection success rate was 90%. The culture temperature was 26°C, the light intensity was 1800lx, and the light was 14 hours / day. Seeds germinate after 28 days.

[0033] The seed germination medium is composed of the following components per liter: 25 g of sucrose, 6 g of agar, and the balance is 1 / 2 MS medium, pH 5.9.

[0034] 2. Induction of adventitious buds: 50 days after seed germination, when the seedling height is 1.5 cm, the hypocotyls are cut and inoculated onto the adventitious bud induction medium to induce adventitious buds. The culture te...

Embodiment 3

[0043] 1. Disinfection method of explants: take the blue-black fruit of Euryodendron excelsum H.T.Chang on the vigorously growing plant, soak in water and remove the pulp to obtain well-developed seeds, first soak in 75% ethanol aqueous solution 30 seconds, then disinfected with a mass fraction of 0.1% mercuric solution for 5 minutes, rinsed 4 times with sterile water, and inoculated into the seed germination medium, and the disinfection success rate was 85%. The culture temperature was 28°C, the light intensity was 2000lx, and the light was 12 hours / day. Seeds germinate after 25 days.

[0044] The seed germination medium is composed of the following components per liter: 30 g of sucrose, 6 g of agar, and the balance is 1 / 2 MS medium, pH 6.0.

[0045] 2. Induction of adventitious buds: 60 days after seed germination, when the seedling height is 2 cm, cut the hypocotyl and inoculate it on the adventitious bud induction medium to induce adventitious buds. The culture temperatu...

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PUM

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Abstract

The invention discloses a method for culturing bloodwood tissue. The present invention uses the hypocotyls of the blood tree seeds as explants to successfully carry out tissue culture and rapid propagation of the high-quality seedlings of the blood tree of the excellent strain, and the produced seedlings of the blood tree have high genetic stability, Rapid emergence, strong seedlings, good seedling quality, strong resistance, good growth and other characteristics; it has a good market prospect.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a method for culturing porcine blood wood tissue. Background technique [0002] Euryodendron excelsum H.T.Chang, the only species of Euryodendron excelsum H.T.Chang, is a perennial woody plant, currently only produced in Bajia Town, Yangchun County, Guangdong Province. Pig's blood wood is a kind of precious wood, and it is also a beautiful ornamental garden seedling. Due to human interference and the destruction of the living environment, it is on the verge of extinction. At present, it is only distributed in Bajia Town, Yangchun County, Guangdong Province, and the number does not exceed 100 plants were listed as national second-class protected endangered plants in 1992, listed as critically endangered by the International Plant Protection Association (IUCN) in 1998, and listed as a national extremely small population wild plant in 2012. It is included in the protection catego...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02P60/40
Inventor 曾宋君熊玉萍文香英张雪莲陈双艳杨科明吴坤林房林李琳马国华
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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