Classification and identification method for evergreen and deciduous euscaphis japonica molecular markers

Abstract

The invention relates to a classification and identification method for evergreen and deciduous euscaphis japonica molecular markers. The method comprises the following steps: collecting populations such as national evergreen euscaphis japonica, deciduous euscaphis japonica and Jianning euscaphis japonica for reduced-representation genome sequencing to obtain SNPs; and utilizing MEGA X software to construct Neighbor-joining on the basis of the SNPs. The result of evolutionary trees shows that the evergreen euscaphis japonica and the deciduous euscaphis japonica are sister populations, the Jianning euscaphis japonica populations are merged into the deciduous euscaphis japonica, evolutionary branching of the euscaphis japonica located at the end of the deciduous euscaphis japonica is realized, and the result can confirm that the previous assumption that the Jianning euscaphis japonica is considered to be the variant of the euscaphis japonica is correct; and the evergreen euscaphis japonica is euscaphis konishii hayata. The good method is provided for scientific classification of euscaphis or scientific planting zoning in production practice, and serious loss caused by mixed planting can be effectively prevented.

Description

technical field [0001] The invention relates to a method for classifying and identifying plant molecular markers, in particular to a method for classifying and identifying molecular markers for evergreen and deciduous species of Arvenia japonica. Background technique [0002] We consulted relevant documents such as flora of various places, "Flora of China", "Floral of China", and found that all recorded Corvus genus ( Euscaphis ) species are deciduous trees or shrubs. In 1784, Thunberg discovered a shrub of the Lonicera family Elderberry in Japan, named Sambucus japonica . Siebold and Zuccarini described a new species in 1835 - Euscaphisataphyleoides , and in 1840, a new genus of the province's Oleaceae was created——Corona genus Euscaphis . In 1878, Kanitz argued that Euscaphisstaphyleoides and Sambucus japonica for the same species, and named it Euscaphis japonica , this species is a deciduous small tree or shrub, with pinnately compound leaves, finely serrated...

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Problems solved by technology

[0005] The team where the invention is located collected more than 350 samples of wild and artificially cultivated populations of Corvus japonica in the main production areas of the country, and completed the sequencing assembly and annotation of the whole genome. Through molecular biological clock analysis, it was found that the genus Corvus vulgaris was about 108 million years ago diverged, and the species divergence of evergreen and deciduous Corvus was about 2 million years ago, although this method is still not very practical

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