Three-freedom-degree pipetting device of closed card box and closed card box

Abstract

The invention provides a three-freedom-degree pipetting device of a closed card box and the closed card box. The freedom degree of the pipetting device is expanded so that more reagent holes can be used in the limited volume of the closed card box. The closed card box comprises a reagent disc, and a plurality of reagent holes are formed in the reagent disc. The pipetting device comprises a pipetting head, a rotating platform, a first driving assembly and a second driving assembly. The rotating platform comprises a rotating base plate and a base plate bearing part, the rotating base plate is rotatably arranged on the base plate bearing part, a sliding groove is formed in the rotating base plate, and the pipetting head is slidably arranged in the sliding groove. The first driving assembly is used for driving the pipetting head to slide along the sliding groove and driving the rotating base plate to rotate relative to the base plate bearing part so as to drive the pipetting head to rotate so that the pipetting head can be aligned with any reagent hole in the reagent disc. The second driving assembly is used for driving the rotating platform to move up and down and driving the pipetting head to move up and down so that the pipetting head can perform liquid suction or liquid injection operation on the aligned reagent hole.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a three-degree-of-freedom pipetting device of a closed cartridge and the closed cartridge. Background technique [0002] Nucleic acid is the main carrier of life genetic information. DNA and RNA sequences are called genetic codes, which are the basis for analyzing gene structure, function and their relationship, as well as the most accurate basis for molecular diagnosis of clinical diseases. Gene sequencing is a new type of gene detection technology, which is also of great significance and value to clinical medicine. Mechanism research and clinical diagnosis of many diseases gradually rely on gene sequencing technology. Before gene sequencing, the sequencing samples need to be prepared. Initially, most preparations were performed manually by humans, but as the number of samples increased, so did the need for automation. [0003] There are two main types of existing aut...

AI Technical Summary

Problems solved by technology

[0004] In related closed cartridges, a two-degree-of-freedom pipetting device is usually used, wherein one degree of freedom is realized by the up-and-down movement of the pipette, and the other degree of freedom is realized by the rotation of the reagent disc. As a result, only one ring of reagent wells can be effectively used in the closed cartridge with limited volume, which greatly limits the number of reagent wells and leads to low preparation efficiency.

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