Method for simultaneously identifying short-cut denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology
A technology of stable isotope and denitrifying bacteria is applied in the field of active short-range denitrifying bacteria and triclosan degrading bacteria, which can solve the problems of unclear relationship, and achieve the effect of improving the identification rate and the distribution ratio.
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Embodiment 1
[0033] A method for simultaneously identifying short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology, specifically comprising the following steps:
[0034] (1) Start-up of a short-cut denitrification system based on granular sludge and the biological removal of triclosan in this system. Set the concentration of sodium propionate and nitrate nitrogen in the influent to 315mg / L and 105mg / L respectively, inoculate aerobic granular sludge with a sludge concentration of 6000mg / L, and start a short-range denitrification sequence batch biological system with an effective volume of 3L. The reactor achieved a stable nitrous accumulation rate after 65 days of operation, and then added 3mg / L triclosan to the system for a total of 130 days of operation. ) to carry out the mass conservation experiment of triclosan to explore the removal of triclosan in the short-range denitrification system. The results of the mass cons...
Embodiment 2
[0046] A method for simultaneously identifying short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology, the specific steps are basically the same as in Example 1, the difference is: step (1) and (2) in Sodium acetate was changed to the corresponding sodium acetate.
Embodiment 3
[0048] A method for simultaneously identifying short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology, the specific steps are basically the same as in Example 1, the difference is: in steps (1) and (2) Sodium propionate was changed to the corresponding methanol.
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