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Application of platymonas selenized exopolysaccharide in lung cancer treatment

A technology of exopolysaccharides and algae selenized exopolysaccharides, which is applied in the application field of exopolysaccharides selenized by algae in the treatment of lung cancer. Effect of unlimited proliferation and promotion of natural apoptosis

Pending Publication Date: 2021-04-02
GUANGDONG ZHANJIANG PROVINCIAL LAB OF SOUTHERN MARINE SCI & ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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[0004] Publication No. CN110272504A titled "Preparation Method of Selenized Phymatomexopolysaccharide and Selenized Phymatomexopolysaccharide" discloses a preparation method of Selenized Phymatomexopolysaccharide and Selenized Phymatomexopolysaccharide, but Application of Undisclosed Selenized Exopolysaccharide of Pyrophytum algae in the Treatment of Lung Cancer

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  • Application of platymonas selenized exopolysaccharide in lung cancer treatment
  • Application of platymonas selenized exopolysaccharide in lung cancer treatment
  • Application of platymonas selenized exopolysaccharide in lung cancer treatment

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Embodiment 1

[0042] Prepare the culture medium with a concentration range of 50-250 μg / μL of the selenized exopolysaccharide of Pyramid algae. After the lung cancer cells are cultured to 80% confluence, culture the cells with the medium containing the selenized exopolysaccharide of Pyramid algae for 24 hours, and then collect the cells And test its cell viability, apoptosis rate and expression of apoptosis gene protein, such as figure 2 shown.

[0043] according to figure 2 , it can be seen that with the increase of the polysaccharide concentration on the abscissa, the inhibition rate of cell proliferation on the ordinate also increases. It can be seen that the increase of the concentration of the selenized exopolysaccharide solution of Phytophthora significantly inhibits the proliferation of A549 cells.

[0044] according to image 3 , it can be seen that with the increase of the intervention time of the abscissa plank algae selenized exopolysaccharide solution on the proliferation of...

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Abstract

The invention discloses an application of platymonas selenized exopolysaccharide in lung cancer treatment. The application process comprises the following steps: culturing lung cancer cells until 80%of the lung cancer cells are fused, performing subculture, selecting subcultured passage cells, culturing the cells with a cell culture solution containing the platymonas selenized exopolysaccharide,and finally, performing cell proliferation, cell apoptosis and apoptosis-related protein expression experiment comparison detection on an experimental group and a control group. The intervention of aplatymonas selenized exopolysaccharide solution inhibits the proliferation of lung cancer cells, successfully inhibits rapid proliferation of the lung cancer cells, obviously increases the apoptosis phenomenon of the lung cancer cells, successfully promotes the natural apoptosis of the lung cancer cells, and inhibits the infinite proliferation of the lung cancer cells, the protein expression levelof the Bcl-2 gene can be remarkably reduced, the protein expression level of the Bax and Caspase-3 genes is up-regulated, and apoptosis of the lung cancer cells is induced.

Description

technical field [0001] The invention relates to the technical field of lung cancer cell therapy, in particular to the application of a selenized extracellular polysaccharide of flat algae in the treatment of lung cancer. Background technique [0002] As one of the largest public health problems in the world, lung cancer is a great threat to human health. Malignant lung cancer is no longer just a serious disease in developed industrial countries. Developing countries are facing a greater burden of disease. The prevention and treatment of lung cancer is particularly important. important. [0003] The A549 cell line is an ideal tumor model commonly used in the study of lung cancer in vitro. At the same time, normal lung epithelial cells Bease-2B cells were used as a normal control to analyze the effect of selenized exopolysaccharides of P. algae in the treatment of lung cancer. Flatweed is a characteristic microalgae species in the Beibu Gulf. It is easy to cultivate and has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P35/00
CPCA61K31/715A61P35/00
Inventor 吴斌华梁少华温俊昊刘梓茂罗辉黄永梅
Owner GUANGDONG ZHANJIANG PROVINCIAL LAB OF SOUTHERN MARINE SCI & ENG
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