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Novel pretreatment method through steam explosion-ammonium sulfite combined pretreatment with high efficiency, cleanness and no inhibitors for enzymatic fermentation

A fermentation inhibitor and pretreatment technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of destroying straw tissue, straw structure, fiber wall breaking, and failure to remove inhibitors, etc., to improve germination and growth, reducing the effect of inhibitor content

Pending Publication Date: 2021-03-23
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The steam explosion pretreatment method provided by the patent CN103103220A only destroys the tissue and straw structure of the straw, but does not remove the inhibitors that affect the enzymatic fermentation
Patent CN109868300A provides hydrothermal + ammonium sulfite to treat straw fibers, and adds alkali to degrade lignin, but it does not achieve the goal of breaking the cell structure of fibers, and the black liquor produced at the same time has pollution problems to the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The crop stalks were evenly processed into small sections of about 3 cm with a guillotine, mixed with 8% (w / w) ammonium sulfite solution, and the pH was adjusted to 8 with 3% ammonia water. Immediately put it into the steam explosion tank, and after 0.8 MPa and 10 minutes, the pressure was released instantaneously to obtain the steam explosion-ammonium sulfite combination pretreated straw. Fully wash with 1 times the volume of distilled water, the chemical humic acid obtained from the steam-exploded straw washing liquid contains 3% according to the humic acid determination method, and the concentration of humic acid is verified to be within 0.5% (w / w) through plant pot experiments Promotes seed germination and growth. The fully washed steam-exploded straw is inserted into Saccharomyces cerevisiae cells according to the solid-to-liquid ratio of 1:8 (w / w), the cellulase load of 25FPU / g, and 5% (w / w) of the system, and cultured at 50°C for 60 hours Solid-state synchronous...

Embodiment 2

[0015] The crop stalks were evenly processed into small sections of about 3 cm with a guillotine, mixed with 8% (w / w) ammonium sulfite solution, and the pH was adjusted to 8 with 3% ammonia water. Immediately put it into the steam explosion tank, and after 0.8MPa and 15 minutes, the pressure was released instantaneously to obtain the steam explosion-ammonium sulfite combination pretreated straw. Fully wash with 2 times the volume of distilled water, the chemical humic acid obtained from the steam-exploded straw washing liquid contains 3% according to the humic acid determination method, and the concentration of humic acid is verified to be within 0.5% (w / w) through plant pot experiments Promotes seed germination and growth. The fully washed steam-exploded straw is inserted into Saccharomyces cerevisiae cells according to the solid-liquid ratio of 1:6 (w / w), the cellulase load of 20FPU / g, and 5% (w / w) of the system, and cultured at 50°C for 60 hours Solid-state synchronous sac...

Embodiment 3

[0017] The crop stalks were evenly processed into small sections of about 3 cm with a guillotine, mixed with 10% (w / w) ammonium sulfite solution, and the pH was adjusted to 9 with 3% ammonia water. Immediately put it into the steam explosion tank, and after 1.0 MPa and 8 minutes, the pressure was released instantaneously to obtain the steam explosion-ammonium sulfite combination pretreated straw. Fully wash with 2 times the volume of distilled water, the chemical humic acid obtained from the steam-exploded straw washing liquid contains 3% according to the humic acid determination method, and the concentration of humic acid is verified to be within 0.75% (w / w) through plant pot experiments Promotes seed germination and growth. The fully washed steam-exploded straw was inserted into Saccharomyces cerevisiae cells according to the solid-to-liquid ratio of 1:4 (w / w), cellulase load of 20FPU / g, and 5% (w / w) of the system, and cultured at 50°C for 72 hours Solid-state synchronous s...

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Abstract

According to the invention, aiming at problems that a straw solid matrix structure is compact and an inhibitor for inhibiting enzymatic fermentation is generated in a pretreatment process in a processfor bioconversion of straw to ethanol, crop straws are pretreated by utilizing advantages of a steam explosion and ammonium sulfite combined pretreatment technology so as to destroy a cell wall structure to expose fiber reaction sites, and lignin and ammonium sulfite are subjected to sulfonation reaction in the pretreatment process to form water-soluble sulfonate, so that aims of breaking walls and removing inhibitors are fulfilled. The method not only helps to reduce inhibitors and improve efficiency of enzymatic fermentation, but also can quickly and effectively obtain chemical humic acid and improve germination and growth of plant seeds.

Description

technical field [0001] The invention belongs to the field of comprehensive utilization of lignocellulosic waste, and in particular relates to a combined steam explosion-immonium pretreatment technology. Background technique [0002] During the reaction process of steam explosion pretreatment of lignocellulosic raw materials, a large amount of phenolic acids produced will inhibit the normal metabolism of microorganisms during solid-state fermentation. Most of these phenolic inhibitors are derived from the degradation of lignin, and a few are the degradation products of hemicellulose. Therefore, how to remove lignin in the pretreatment process to reduce the inhibition of subsequent solid-state fermentation is extremely important. Ammonium sulfite pulping is a commonly used method in the paper industry, using SO 3 2- 、HSO 3 - A sulfonation reaction occurs with lignin, which destroys lignin macromolecules and degrades them to form lignosulfonate with higher hydrophilicity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C05F11/02C12R1/865
CPCC12P7/10C12P19/14C05F11/02C12P2201/00Y02E50/10
Inventor 陈洪章赵启红王岚
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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