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Flammulina velutipes strain and cultivation method thereof

A cultivation method, the technology of Flammulina velutipes, which is applied in the fields of mushroom cultivation, cultivation, plant cultivation, etc., can solve the problems of few strains, low yield, poor commerciality of Flammulina velutipes, etc., and achieve the effects of good palatability, less fluff, and short transition period

Active Publication Date: 2021-03-23
河北省农林科学院经济作物研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome defects such as poor commerciality of Flammulina velutipes in the prior art, few strains, low yield, thereby providing a kind of Flammulina velutipes bacterial strain and its cultivation method

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  • Flammulina velutipes strain and cultivation method thereof

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Embodiment 1

[0050] A Flammulina velutipes strain, Jijin 12, is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, address: Institute of Microbiology, Chinese Academy of Sciences, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCCNo.19644, and the preservation date is May 26, 2020. Classification and naming of Jijin 12: silk-covered mushrooms, Flammulina filiformis.

[0051] The breeding method of above-mentioned Flammulina velutipes bacterial strain, comprises the following steps,

[0052] Inoculate the mycelium of Flammulina velutipes strain HUANG1 in an Erlenmeyer flask filled with liquid medium, culture it statically at 25°C for 8 days, and shake the flask by hand every 24 hours. Mycelium, with a mass concentration of 2% lysozyme enzyme solution (purchased from Guangdong Provincial Institute of Microbiology), enzymolysis at 30 ° C for 4 hours, filtered off broken mycelium, washed 3 t...

Embodiment 2

[0074] The cultivation method of Flammulina velutipes strain Jijin 12 comprises the following steps:

[0075] (1) Propagation of the mother species: inoculate Jijin 12 strains with a diameter of 0.3-0.5 cm on the culture medium of the mother species, and cultivate them under conditions of 21±1°C, relative air humidity of 45±5%, and dark conditions for 7 days. 12 days, obtain Jijin 12 parent species; Wherein, the mass ratio of Jijin 12 strains and parent species culture medium is 1:100; The preparation method of mother species culture medium comprises, 200g peeled potato dicing is placed in 600ml water, Boil for 30 minutes, filter and take the filtrate, add 400ml of water, 20g of glucose and 20g of agar to the filtrate, set the volume to 1000ml, heat on low heat while stirring until the above ingredients are completely dissolved, then sterilize at 121°C for 30 minutes, and set aside.

[0076] (2) Original seed culture: Inoculate the Jijin 12 female seed in the original seed med...

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Abstract

The invention belongs to the technical field of edible mushrooms, and particularly relates to a flammulina velutipes strain and a cultivation method thereof. The pileus of the strain is golden yellow,bright in colour and thin in stipe; the average diameter of the stipe is about 0.25 cm; the stipe is yellowish white and not prone to browning; the stipe base is little in fluff and not sticky; aftertimely harvesting, the average lignin content of stipes is not higher than 2.50*10<3>A280nm / kg and is obviously lower than the lignin content (3.05*10<3>A280nm / kg) of the traditional variety throughdetermination; commercial mushrooms are good in palatability and suitable for fresh eating; Jijin 12 is suitable for an agronomic facility cultivation mode; the single-tide single-bag fruiting yield can reach 288+ / -36.5 g; three-tide mushrooms can be produced through a mushroom intertidal-stage water supplementing fruiting technology; and the average total biological efficiency ranges from 120-140%.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to a Flammulina velutipes strain and a cultivation method thereof. Background technique [0002] Flammulina velutipes (Flammulina filiformis) It belongs to the genus Shiitake, and its Chinese name is Sigaidonggu. It has been used before F. velutiper (Fr) Sing This Latin scientific name, until 2018, Yang Zhuliang of the Kunming Institute of Botany, Chinese Academy of Sciences, and his team rectified the name of Chinese Flammulina velutipes. Research has proved that Chinese Flammulina velutipes is different from European and American F. velutiper , is a new species and should adopt F. filiformis This Latin name. Flammulina velutipes is rich in a variety of nutrients, because it is rich in "lysine", it is also called "education mushroom". Flammulina velutipes are divided into yellow varieties and white varieties, yellow is the wild type, and white is the mutant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G18/00A01G18/20C12R1/645
CPCC12N1/14A01G18/00A01G18/20
Inventor 李慧夏伟伟张晓雅高春燕王朝江董庆武
Owner 河北省农林科学院经济作物研究所
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