Fluorescence immunochromatography test strip capable of detecting early infection of pig trichina and production method thereof

Abstract

The invention discloses a fluorescence immunochromatography test strip capable of detecting early infection of pig trichina and a production method thereof, and belongs to the technical field of serological detection. In order to detect trichina infection more quickly, conveniently and sensitively, the test strip comprises a sample pad, a combination pad, a chromatography membrane, a water absorption pad and a bottom plate, and a fluorescent probe is sprayed on the combination pad. The fluorescent probe comprises a time-resolved fluorescent microsphere coupled goat anti-rabbit IgG serving as an indication probe and a time-resolved fluorescent microsphere coupled trichina ES mixed antigen serving as a capture probe, wherein the trichina ES mixed antigen consists of a trichina muscle larva excretion secretion ML-ESP antigen and a trichina intestinal muscle larva excretion secretion iML-ESP antigen; and a detection line and a quality control line are arranged on the chromatography membrane, mouse anti-pig IgG is sprayed on the detection line, and rabbit anti-goat IgG is sprayed on the quality control line. The test strip can be used for on-site rapid detection of trichina.

Description

technical field [0001] The invention belongs to the technical field of serological detection, and in particular relates to a fluorescent immunochromatographic test strip capable of detecting early infection of trichinella spiralis in pigs and a preparation method thereof. Background technique [0002] Trichinellosis caused by Trichinella spiralis is an important zoonotic parasitic disease, which can infect more than 150 carnivores and omnivores. People mainly eat raw or half-raw meat and meat products containing Trichinella muscle larvae And infection, at the same time only human infection shows obvious clinical symptoms. According to the Trichinella International Committee report: during 1986-2009, a total of 65818 cases of Trichinella infection occurred worldwide. At the same time, according to relevant literature reports, from 1964 to 2011, there were 38,794 cases of Trichinella infection in my country, of which 336 patients died. Therefore, my country has become one of...

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Problems solved by technology

The patent with the application number of 201910604044.8 as the prior art provides a trichinellosis fluorescent immunochromatographic detection test strip, which includes a sample pad, a binding pad, a chromatographic membrane, a water-absorbing pad and a bottom plate. The binding pad is marked with time-resolved fluorescent microspheres coupled with goat anti-pig IgG; the chromatographic membrane is provided with a detection line and a quality control line, wherein the detection line is sprayed with ML-ESP antigen and Trichinella spiralis muscle larvae excreta excretion iMP-ESP antigen composed of Trichinella spiralis muscle larvae ES mixed antigen; the quality control line is sprayed with rabbit anti-goat IgG, this test strip is consistent with the ES ELISA method stipulated by OIE Although it can accurately detect positive trichinella spiralis antibodies earlier after infection with trichinella spiralis and shorten the "window" period of detection, the earliest time point when the test strip can detect positive positive antibodies for trichinella spiralis is from the development of trichinella spiralis It is still some time before the infective larvae with the ability to infect are formed, so the technology needs to be further improved

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