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DNA methylation markers, diagnostic reagents and application of psoriatic arthritis

A technology for methylation and arthritis, which is applied in the field of medical molecular biology detection, can solve the problems of early diagnosis of patients without PsA, and achieve the effect of convenience and easy development, good application prospects, and less blood samples

Active Publication Date: 2022-06-28
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the current diagnosis is mainly based on the patient's medical history, clinical manifestations, and joint X-ray changes, and there are currently no biological diagnostic markers for early diagnosis of PsA patients

Method used

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  • DNA methylation markers, diagnostic reagents and application of psoriatic arthritis
  • DNA methylation markers, diagnostic reagents and application of psoriatic arthritis
  • DNA methylation markers, diagnostic reagents and application of psoriatic arthritis

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Experimental program
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Embodiment

[0068] 1. Screening of DNA methylation CpG sites

[0069] Whole blood samples from 25 patients with psoriatic arthritis and 19 healthy controls were detected by whole blood genome 850k methylation chip, and a batch of DNA methylation differentially expressed between psoriatic arthritis and healthy controls was initially screened out. CpG sites.

[0070] 2. Identification of DNA methylation CpG sites

[0071] Through the design of primers, PCR amplification of target gene bands, pyrosequencing and other experiments. Finally, the differentially expressed DNA methylation CpG sites were screened and identified for the diagnosis of PsA.

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Abstract

The invention discloses a DNA methylation marker for psoriatic arthritis (PsA), a diagnostic reagent and applications thereof. Including 6 methylation sites: one or more of chr14:38061326, chr14:38061320, chr9:128585454, chr6:37225002, chr3:101901234, chr6:97369501. The present invention uses specific DNA methylation sites as molecular markers for the first time to assist in the screening of psoriatic arthritis and rheumatoid arthritis (Rheumatoid Arthritis, RA) patients, opening up a new way for diagnosis in this field and way. The invention utilizes whole blood of patients to detect DNA methylation sites to achieve the purpose of early diagnosis of PsA, requires less blood samples, is convenient and easy to carry out, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of medical molecular biology detection, and particularly relates to a diagnostic marker, a diagnostic reagent and an application of psoriatic arthritis. Background technique [0002] Psoriatic arthritis (PsA) is an inflammatory joint disease associated with psoriasis that has a psoriatic rash and causes joint and surrounding soft tissue pain, swelling, tenderness, stiffness, and movement disorders. Some patients may have sacroiliitis and / or spondylitis. The course of the disease is protracted, prone to recurrence, and joint ankylosis may occur in the late stage, resulting in disability. The peak age of onset of PsA is 35-45 years old. About 70% of PsA patients have psoriatic skin lesions before joint inflammation, and about 15% of patients have skin lesions and joint inflammation at the same time. Early PsA refers to the absence of pain symptoms within 1-2 years after the patient is diagnosed. Therefore, it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6869C12N15/11
CPCC12Q1/6883C12Q2600/158
Inventor 陆前进邓敏赵明苏玉文吴海竞
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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