Kit for simultaneously detecting streptococcus pneumoniae, legionella pneumophila and Moraxella catarrhalis

Abstract

The invention relates to the technical field of fluorescent PCR detection, in particular to a kit for simultaneously detecting streptococcus pneumoniae, legionella pneumophila and Moraxella catarrhalis. A fluorescent PCR detection system comprises primers of specific conserved sequences of streptococcus pneumoniae, legionella pneumophila and Moraxella catarrhalis and a TaqMan fluorescent probe, and the DNA sequence of the fluorescent PCR detection system is SEQ ID NO: 1-9. The kit has the advantages that multiple fluorescent quantitative PCR detection technologies are utilized, the purpose ofsimultaneously detecting three respiratory tract pathogens including streptococcus pneumoniae, legionella pneumophila and Moraxella catarrhalis is is achieved, the detection method is rapid and accurate, timeliness, specificity and sensitivity of detection are guaranteed, and the kit has important application value in the fields of disease monitoring, clinical diagnosis and the like.

Description

technical field [0001] The invention relates to the technical field of fluorescent PCR detection, in particular to a kit for simultaneously detecting Streptococcus pneumoniae, Legionella pneumophila and Moraxella catarrhalis. Background technique [0002] Respiratory tract infection is one of the most common infectious diseases worldwide and an important issue affecting human health. Respiratory tract infection includes upper respiratory tract infection and lower respiratory tract infection. There are many pathogens that can cause respiratory tract infection, including bacteria, viruses, mycoplasma, chlamydia, etc. The symptoms caused by these pathogens are similar, and it is difficult to distinguish them based on clinical symptoms. Lower respiratory tract infection refers to the inflammation of the terminal airways, alveoli and pulmonary interstitium, which can be caused by disease microorganisms, physical and chemical factors, immune injury, allergies and drugs. Among the...

AI Technical Summary

Problems solved by technology

The traditional method of isolation without culture needs to be cultured and isolated to identify the strain, which takes a long time, and the growth conditions of Legionella pneumophila are harsh, the nutritional requirements are high, time-consuming and labor-intensive, it is not conducive to rapid detection, and it has certain requirements for operators. risk, thus limiting its clinical application

The immunoassay detection reagent is aimed at the antigen in the sample or the specific antibody produced by the body caused by the pathogen, and there is a window period for antibody detection, so it is easy to miss the diagnosis in the early stage of the disease

In addition, although the serological method is mature and commercial kits are available, the sensitivity and specificity need to be improved. At the same time, it is necessary to detect double serum in the acute phase and the convalescent phase to be diagnostically meaningful and it is easy to miss the diagnosis.

However, the fluorescent PCR detection in the prior art is difficult to realize multiple detection of SP, LP, and MC due to reasons such as primer specificity.

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