Detection method of HLA-B*1502 gene, detection kit and application thereof
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A technology for HLA-B and detection reagents, applied in biochemical equipment and methods, microbial measurement/testing, recombinant DNA technology, etc., can solve problems such as time-consuming, high cost, and cumbersome operations
Active Publication Date: 2021-03-12
上海恩元生物科技有限公司
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Problems solved by technology
However, while having advantages, there are also disadvantages such as cumbersome operation, long time-consuming, high cost, etc.
In particular, the shortcomings of cumbersome operation and long time consumption can no longer meet the needs of clinical medical testing, and cannot adapt to the "fast and simple" concept required by clinical medical testing.
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Embodiment 1
[0063] In this embodiment, specifically, a corresponding primer group and kit, which can be used for a genoty population of patients with epilepsy patients.
[0064] This embodiment provides a detection agent for HLA-B * 1502 gene type detection.
[0065] The detection reagent comprises: a nucleic acid amplification system, the kit nucleic acid amplification system comprising a MIX1 reaction solution and a primer 1 and primer 1 for amplifying oligonucleotide nucleic acids, primer 7 and primer 8; MIX2 reaction liquid and Primers 3 and primers 4, primers 7 and primers 8; MIX3 reaction fluids and primers 6 for amplifying oligonucleic acid nucleic acids, primers 6, primers 7 and primers 8; The nucleotide sequences of the primer 1-primer 8 are respectively shown in SEQ ID NO.1-SEQ ID NO. (Table 1), respectively.
[0066]
[0067] The detection reagent also includes a fluorescence detection system, which is used to include fluorescence detection probe 1 and probe 4, MIX2 reaction liqu...
Embodiment 2
[0099] In this embodiment, by the case of some epilepsy patients, a PCR-SBT kit is used as a gold-standard HLA-B * 1502 detecting method, respectively, and the detection reagent, corresponding kit and corresponding method, corresponding kit, and corresponding method. Sampling, detecting HLA-B * 1502 sites, and gene-profiling test results are shown in Table 8.
[0100]
[0101] The above results can be seen that the resulting result of the genotyping result obtained by the kit of Example 1 and the consistency of the gene-based generated result obtained by the PCR-SBT kit was 100%.
[0102] It can be seen that the detection of the detection reagent, the corresponding kit and method is performed using the detection reagent of Example 1, and the detection result of the HLA-B * 1502 site is reliable, and the detection reagent and corresponding kit and the corresponding kit are used. The method can accurately detect the human leukocyte antigen B site 1502 gene, so it can accurately us...
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Abstract
The invention belongs to the technical field of biology, and provides a reagent for detecting human leukocyte antigen B site 1502 genotype (HLA-B*1502), application of the reagent in preparation of akit for guiding administration of antiepileptic drugs such as carbamazepine, oxcarbazepine, phenytoin and lamotrigine, a corresponding kit and a detection method of the kit. According to the HLA-B*1502 genotyping, three groups of MIX reaction solutions are detected by adopting a PCR-fluorescent probe method, and target nucleic acid molecules are circularly amplified through an amplification reaction, so that a fluorescence generation group is indirectly combined with an amplified target nucleotide sequence; the amount of fluorescence generated by the fluorescence generation group is determined, and the existence of the target nucleotide is determined. The detection reagent comprises a nucleic acid amplification system of three groups of MIX reaction solutions, wherein the nucleic acid amplification system comprises an upstream primer 1 and a downstream primer 1 which can be combined with target nucleotide, and an upstream primer 2 and a downstream primer 2 which can be combined with the target nucleotide; and three groups of probes 1 and probes 4 of a fluorescence detection system matched with the nucleic acid amplification system.
Description
Technical field [0001] The present invention belongs to the field of biotechnology, and more particularly to human white blood cell antigen B sites for human leuvisulphic antigenic drug medication guidance in Kamasipine, Okasi, phenytoin, Ramamazine (HLA-B * 1502) Detection reagents, detection methods and kits. The present invention also includes the use of the above-described detecting reagent in the preparation of the kits for epileptic patients to take cardaasi, Okazi, phenytoin, mamitastic treatment, and the corresponding kit and the use of the same. Background technique [0002] Mental illness has become a global high-haired disease. According to statistics, more than 17% of my country's adults were deeply plagued by mental illness such as schizophrenia and epilepsy (Phillips.2009). Epilepsy is defined by the World Health Organization (WHO) and the International Anti-epileptic Union (ILA) as: a chronic brain disease caused by multiple causes, mainly due to the repeated and e...
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