Composite culture medium for rapidly detecting total number of moulds and saccharomycetes and preparation method thereof

A technology of culture medium and yeast, which is applied in the field of compound culture medium for rapid detection of the total number of mold and yeast, can solve the problems of long time-consuming, detection rate needs to be improved, etc., and achieve the effect of easy counting and large colony shape

Pending Publication Date: 2021-03-12
NANJING ZHONGKE GRP CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection medium for the total number of molds and yeasts is usually potato agar or Bengal red agar. Most molds and yeasts can only form all colonies that can be distinguished by naked eyes after being cultured on these two agars for 120 hours, which takes a long time. Detection rate needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take casein trypsin digest 0.9g, glucose 3.0g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.1g, yeast extract 0.6g, Bengal red 0.6g, agar 2g, chloramphenicol 0.1g, magnesium sulfate 0.3 g, manganese chloride 0.02g, calcium chloride 0.1g, Tween 600.2g. Add the above components into 100 g of distilled water, boil and dissolve to a clear solution, adjust the pH value to 7.0, and autoclave at 115°C for 15 minutes before use.

Embodiment 2

[0022] Casein trypsin digest 0.8g, glucose 4.0g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.2g, yeast extract 0.4g, Bengal red 0.4g, agar 3g, chloramphenicol 0.1g, magnesium sulfate 0.2g , manganese chloride 0.03g, calcium chloride 0.05g, Tween 600.25g. Add the above components into 100 g of distilled water, boil and dissolve to a clear solution, adjust the pH value to 7.0, and autoclave at 115°C for 15 minutes before use.

Embodiment 3

[0024] Casein trypsin digest 1.0g, glucose 1.0g, potassium dihydrogen phosphate 0.3g, sodium chloride 0.05g, yeast extract 0.8g, Bengal red 0.8g, agar 1.5g, chloramphenicol 0.2g, magnesium sulfate 0.4 g, manganese chloride 0.015g, calcium chloride 0.2g, Tween 60 0.1g. Add the above components into 100 g of distilled water, boil and dissolve to a clear solution, adjust the pH value to 7.0, and autoclave at 115°C for 15 minutes before use.

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PUM

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Abstract

The invention relates to a composite culture medium for rapidly detecting total number of moulds and saccharomycetes. Every 100 g of distilled water in the composite culture medium contains 0.8-1.0 gof casein pancreatin digests, 1.0-4.0 g of glucose, 0.2-0.3 g of potassium dihydrogen phosphate, 0.05-0.2 g of sodium chloride, 0.4-0.8 g of a yeast extract, 0.4-0.8 g of rose bengal, 1.5-3 g of agar,0.1-0.2 g of chloramphenicol, 0.2-0.4 g of magnesium sulfate, 0.015-0.03 g of manganese chloride, 0.05-0.2 g of calcium chloride, and 0.1-0.25 g of Tween 60. By adopting the composite culture medium,the total number of molds and saccharomycetes in various foods can be rapidly detected, the detection rate is 20% higher than that of potato agar and rose bengal agar, the bacterial colony morphologyis large, counting is easy, and bacterial colonies are basically formed and basically grow completely within 72 hours.

Description

technical field [0001] The invention belongs to the technical field of microbiological detection, and in particular relates to a compound culture medium for rapidly detecting the total number of molds and yeasts. Background technique [0002] The total number of molds and yeasts is the total number of microbial molds and yeasts formed in each g (ml) of the sample obtained after the food samples are cultured under certain conditions (such as culture medium, culture temperature and culture time, etc.). Mold and yeast widely exist in nature. Some molds can also be used to make food, but some molds are harmful. They can cause food mildew, irritate the human digestive tract, and can be poisonous and damage the liver. [0003] At present, the detection medium for the total number of molds and yeasts is usually potato agar or Bengal red agar. Most molds and yeasts can only form all colonies that can be distinguished by naked eyes after being cultured on these two agars for 120 hour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12N1/14C12N1/16C12R1/645
CPCC12Q1/06C12N1/14C12N1/16
Inventor 韩丰成凡莉施巍巍胡浪钱一帆
Owner NANJING ZHONGKE GRP CORP LTD
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