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High-enzyme-activity modified membrane and preparation method thereof

A high-activity, high-enzyme technology, applied in the direction of coating, etc., can solve the problems of reduced flexibility and activity, easy leakage of enzymes, poor stability, etc., to achieve high enzyme activity, prolong service life, and prolong the effect of cleaning costs

Active Publication Date: 2021-03-12
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of the enzyme will change after the enzyme is immobilized, and the flexibility and activity of the enzyme will be greatly reduced.
At the same time, these physical immobilization methods are less stable, and the enzyme is prone to leakage and loss during the reaction process.

Method used

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  • High-enzyme-activity modified membrane and preparation method thereof
  • High-enzyme-activity modified membrane and preparation method thereof
  • High-enzyme-activity modified membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0040] In this preparation example, an enzyme liquid with high enzyme activity is prepared. Steps include:

[0041] Dilute the pepsin powder with citric acid-sodium hydroxide-hydrochloric acid buffer solution with a pH=3.0 and a concentration of 0.2M to obtain a pepsin solution. After refrigerated at 4°C, it is treated with a pulsed electric field. The electric field strength is 10kV / cm, and the flow rate is 80mL·min -1 , obtain high enzyme activity enzyme liquid, the concentration of pepsin in the high enzyme activity enzyme liquid is 6mg / mL, and the pepsin enzyme activity of high enzyme activity enzyme liquid is 50 (10 3 , U).

preparation example 2

[0043] The difference from Preparation Example 1 is that the electric field strength is 0kV / cm, and the flow rate is 0mL min -1 , the enzyme activity is 30(10 3 , U).

preparation example 3

[0045] The difference from Preparation Example 1 is that the electric field strength is 4kV / cm, and the flow rate is 50mL min -1 , the enzyme activity is 35.5 (10 3 , U).

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Abstract

The invention belongs to the field of preparation of modified membranes, and particularly relates to a high-enzyme-activity modified membrane and a preparation method thereof. The preparation method comprises the following steps of: diluting pepsase powder with a citric acid-sodium hydroxide-hydrochloric acid buffer solution to obtain a pepsase solution, refrigerating the pepsase solution, and treating the pepsase solution with a pulsed electric field to obtain a high-enzyme-activity enzyme solution; soaking a PES membrane in a Tris-HCl buffer solution, adding procyanidine and polyethyleneimine to obtain a mixed solution, performing stirring until the procyanidine and polyethyleneimine are completely coated, and conducting cleaning to obtain the modified membrane; and immersing the modified membrane in the high-enzyme-activity enzyme solution to obtain the high-enzyme-activity modified membrane. The modified membrane with continuous high enzyme activity prepared by the method disclosedby the invention not only has high enzyme activity, but also solves the problems of repeated use capability of enzyme and hydrophilicity and anti-pollution capability of the membrane.

Description

technical field [0001] The invention belongs to the field of modified membrane preparation, and more specifically relates to a modified membrane with high enzyme activity and a preparation method thereof. Background technique [0002] As an emerging non-thermodynamic physical sterilization technology, high-voltage pulsed electric field has been used to regulate the activity and stability of many enzymes due to its advantages of low energy consumption, short-term treatment and no chemical residues, and has achieved significant results. Success, indicating that low-intensity high-voltage pulsed electric fields can increase enzyme activity. However, the enzyme activity after high-voltage pulsed electric field treatment will decrease for a period of time, even lower than that of the untreated enzyme, which will greatly reduce the repeated use of the enzyme. [0003] As a biocatalyst, enzymes are sensitive to the environment and are easily inactivated. Immobilization of enzymes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/056C08J7/04C08L81/08
CPCC08J7/056C08J7/042C08J7/0427C08J2381/08C08J2379/02
Inventor 祝振洲陈哲李书艺
Owner WUHAN POLYTECHNIC UNIVERSITY
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