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Method for improving sensitivity and quantitative analysis capability of miniature mass spectrometer and application

A four-stage mass spectrometer and target technology, which is applied in the field of improving the sensitivity and quantitative analysis capabilities of micro-mass spectrometers, can solve problems affecting the qualitative and quantitative accuracy of ion trap mass spectrometers, so as to improve sensitivity and quantitative analysis capabilities, and expand applications Range, the effect of increasing signal sensitivity

Active Publication Date: 2021-03-05
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

FNF is effective in reducing the impact of space charge effects on dynamic range and resolution, but its generation requires additional hardware
The above-mentioned methods are all linear frequency sweep signals. From the perspective of the relationship between time and frequency, one time point only corresponds to one frequency, and it is impossible to simultaneously remove multiple ions at one time, so its ability to reduce the space charge effect is limited. , which directly affects the qualitative and quantitative accuracy of the ion trap mass spectrometer

Method used

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  • Method for improving sensitivity and quantitative analysis capability of miniature mass spectrometer and application
  • Method for improving sensitivity and quantitative analysis capability of miniature mass spectrometer and application
  • Method for improving sensitivity and quantitative analysis capability of miniature mass spectrometer and application

Examples

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Effect test

Embodiment 1

[0025]Taking a linear ion trap as an example, the mass spectrum instrument uses a frequency scan mode, and the load timing is performed in the injection or other stage by importing the wideband signal generated by MATLAB, and the Grid-Swift and SWIFT are in ion selectivity in the injection or scan. Enrichment effect.image 3 A-C is a mixture of SWIFT and GRID-SWIFT signals in the injection stage, which is a mixture of PEG300 and lesions, wherein the bloodline is a target analyte.image 3 b is an effect of Swift, since the frequency time of SWIFT is single change, only one corresponding frequency ion can only be emitted, and other ions can enter the ion trap, so that the peak of the PEG300 is still Some remains.image 3 C is to apply a Grid-Swift signal in the injection stage, and the impurity peak of the PEG 300 is filtered off, and the target ion-lesion is highlighted.image 3 D-E compares Grid-SWIFT and the effect of implying SWIFT in the ion prisoner. The sample used is a mixed sampl...

Embodiment 2

[0027]The Grid-Swift application is lifted in a single ion monitoring (SIM) mode and selective reaction monitoring, SRM mode. Since Grid-Swift covers a wide frequency range at each time, it is possible to simultaneously pop up non-target ions, selectively accumulate target ions, improve the utilization of well capacity, reduce spatial charge effects, and enhance the sensitivity of the instrument. . This embodiment is used as a PEG400 (1ug / mL) and different concentrations of levity, respectively, separate Swift and Grid-Swift signals, respectively.Figure 4 A is the detection line comparison of the two methods in SIM mode, and the equipage of the Grid-Swift signal detection line is applied higher than the fitting advantage of the application of SWIFT. The target ion M / Z 609 collisions induced dissociation (CID), monitors its characteristic fragment ion M / Z 397, and the detection line fitting optimum optimization of the Grid-Swift signal is greater than the application of SWIFT. ...

Embodiment 3

[0029]Combined with Multiple Reaction Monitoring (MRM) mode, the target is complicated with the target, using MRFA and hypotranside (100 ng / ml) mixed sample, where blood is the internal standard ion, the concentration of MRFA is Change within a certain range,Figure 5 A is the full spectrum of MRFA and the blood.Figure 5 B is an isolated spectrum for the application of Grid-SWIFT signal monitoring target MRFA (M / Z 524) and Lizi (M / Z 609),Figure 5 C is a calibration curve under Multiple Ion Monitoring (MIM), and the concentration of MRFA changes from 5 ng / ml to 1 ng / ml, the ordinate of the response curve is the intensity of the peak m / z 524 and m / z 609. The ratio, within which the calibration curve shows a good linearity. In order to confirm the target monitor, the ions isolated in MIM mode were cid, and the characteristic debris monitors of two targets were selected, respectively from the fragment ions M / Z 271 from MRFA and fragments from the blood plane. Ion M / Z 39...

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Abstract

Firstly the invention provides a multi-ion simultaneous isolation method for a quadrupole mass spectrometer. Compared with a traditional linear sweep frequency isolation method, the method has the advantages that target ions can be enriched more effectively and selectively, the space charge effect caused by interaction between the ions is reduced, and the sensitivity, resolution and accuracy of the instrument are improved. Secondly, the invention provides a method for improving the sensitivity and quantitative analysis capability of the miniature mass spectrometer, selective enrichment of target ions and removal of non-target ions are realized by using the multi-ion simultaneous isolation method, and a pseudo multi-reaction monitoring mode is realized by combining the method with a composite scanning mode, the method is used for quantitative analysis of a target object, and functions and application range of the miniature mass spectrometer.

Description

Technical field[0001]The present invention relates to the field of mass spectrometry, and in particular, to a method and application of a sensitivity and quantitative analysis capability for lifting micro mass spectrometry.Background technique[0002]The micro mass spectrometry is widely used in the field of fast detection, such as environmental testing, food safety, land security, spatial detection, clinical testing, etc. due to its portability. The size reduction is the main target of miniaturization, but it is easy to use to expand the actual factors of micro mass spectrometry. With the development of open ion source technology, micro mass spectrometers are directly analyzed. The combination of open ionospheres and micro mass spectrometry requires an effective atmospheric interface, a non-conneity atmospheric pressure interface (Traontinuous atmospheric pressureinterface, dapi) and continuous atmosphericpressure interface (CAPI) developed by Beijing University of Science and Techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 徐伟姜婷许祖强
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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