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A kind of Lycium barbarum ace, dpp-iv inhibitory peptide and derivative polypeptide and application, mixture

A technology of DPP-IV and inhibitory peptides, applied in application, peptide, drug combination, etc., can solve the problems of liver and kidney tissue burden, toxic side effects, liver and kidney damage, etc.

Active Publication Date: 2021-11-30
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the toxic and side effects of chemically synthesized western medicine antihypertensive drugs on the human body, especially the drug absorption causes a great burden on the liver and kidney tissues, causing liver and kidney damage, and the symptoms recur after drug withdrawal, and the curative effect is not very satisfactory

Method used

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  • A kind of Lycium barbarum ace, dpp-iv inhibitory peptide and derivative polypeptide and application, mixture
  • A kind of Lycium barbarum ace, dpp-iv inhibitory peptide and derivative polypeptide and application, mixture
  • A kind of Lycium barbarum ace, dpp-iv inhibitory peptide and derivative polypeptide and application, mixture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The identification of embodiment 1 polypeptide LLEPIGVVGH

[0017] Lycium barbarum protein was subjected to Bacillus subtilis fermentation, acid precipitation and ethanol precipitation, and LC-MS / MS analysis, and bioactive peptides with inhibitory activity on ACE were screened by bioinformatics and structure-activity relationship.

[0018] The specific method is as follows:

[0019] (1) Preparation of wolfberry protein extract

[0020] Using 10g of dried wolfberry as raw material, weigh wolfberry and add 100mL of deionized water to soak for 2 hours, then pulverize, ultrasonically extract for 60min, centrifuge at 5000rpm for 10min, and 9.6g of precipitate is used to extract wolfberry protein.

[0021] After precipitation and freeze-drying, pulverize again to obtain wolfberry powder, add 192mL of organic solvent extract to the wolfberry powder, n-hexane:ethanol=2.6:1 (v / v), stir and extract at a temperature of 50°C and a rotating speed of 150rpm for 1 hour, filter, filte...

Embodiment 2

[0030] Example 2 Detection of ACE inhibitory activity of Lycium barbarum active peptide LLEPIGVVGH

[0031] Polypeptide LLEPIGVVGH was synthesized by Nanjing Jiepeptide Biotechnology Co., Ltd., the amino acid sequence is Leu-Leu-Glu-Pro-Ile-Gly-Val-Val-Gly-His, it is a single-chain linear structure, white powder, soluble in water, The molecular weight is 1033.24Da.

[0032] Information on SEQ ID No.1

[0033] (a) Sequential features

[0034] *Length: 10 amino acids

[0035] *Type: amino acid

[0036] * Chain type: single chain

[0037] (b) Molecule type: protein

[0038] Sequence description:

[0039] SEQ ID No.1

[0040] LLEPIGVVGH

[0041] N-(3-(2-Furoyl)acryloyl-phenyl-glutamyl-glutamic acid (FAPGG, λmax=340nm, ε=2270M -1 cm -1 , molecular weight 399.40) can be enzymatically hydrolyzed by ACE into N-[3-(furyl)acryloyl]-2-phenylalanine (FAP, λmax=340nm, ε=1512M -1 cm -1 ) and glycylglycyl peptide (GG, no absorption at 340nm), so it can be used as the mimic substr...

Embodiment 3

[0059] Example 3 Detection of DPP-IV inhibitory activity of Lycium barbarum active peptide LLEPIGVVGH

[0060] DPP-IV can degrade glucagon-like peptide-1 (GLP-1) because GLP-1 has an X-Ala structure. For peptides with N-terminal X-Pro and X-Ala, DPP-IV The dipeptide can be selectively cleaved. In this experiment, we chose Gly-Pro-p-nitroanilide instead of GLP-1 as the simulated substrate of DPP-IV. The generated p-nitroaniline has absorption at 405nm, and the inhibitory activity of DPP-IV can be calculated by absorbance.

[0061] The reaction system is:

[0062] (1) Buffer solution: 100 mM Tris-HCl buffer (pH=8.0).

[0063] (2) Substrate solution: Gly-pro-p-nitroanilide solution with a concentration of 1.59 mM was prepared using the above buffer solution.

[0064] (3) Enzyme solution: use the above buffer solution to prepare 0.01 U / mL DPP-IV solution.

[0065] (4) Sample solution: The polypeptide LLEPIGVVGH was prepared with the above-mentioned buffer solution into solutio...

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Abstract

The invention relates to a polypeptide with angiotensin-converting enzyme (angiotensin-converting enzyme, ACE) and dipeptidyl peptidase IV (dipeptidyl peptidase IV, DPP-IV) inhibitory activity prepared by using wolfberry protein through a fermentation method. The polypeptide LLEPIGVVGH has an amino acid sequence of Leu-Leu-Glu-Pro-Ile-Gly-Val-Val-Gly-His and a molecular weight of 1033.24Da. It has good ACE and DPP‑IV inhibitory activity, and has a good application prospect of being developed into a functional food or a drug for regulating blood pressure.

Description

technical field [0001] The present invention relates to a kind of polypeptide LLEPIGVVGH with angiotensin-converting enzyme (angiotensin-converting enzyme, ACE) and dipeptidyl peptidase (dipeptidyl peptidase IV, DPP-IV) inhibitory activity prepared by fermenting Lycium barbarum protein, which regulates Application of functional foods and medicines in blood pressure. Background technique [0002] As one of the most common cardiovascular and cerebrovascular diseases, hypertension is the most important risk factor for stroke, coronary heart disease and other cardiovascular diseases. Long-term hyperglycemia can cause pathological changes in various tissues and organs throughout the body, leading to acute and chronic complications, such as dehydration and electrolyte imbalance. With the rapid development of social economy and the change of people's living and dietary structure, the incidence of high blood pressure and high blood sugar is also increasing year by year, seriously a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P9/12A61P3/10A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P3/10A61P9/12C07K7/06A23V2200/326A23V2200/328
Inventor 靳艳庄晶玲叶明亮
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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