Application of a bile acid compound bacterial agent in the preparation of mutton sheep feed additive
A technology of feed additive and compound bacterial agent, applied in the field of poultry breeding, can solve the problems of reduced ewe feeding amount, lack of nutrition, shortage of sheep source, etc., and achieves the improvement of slaughter weight and slaughter rate, broad application prospects, and improved breeding income. Effect
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Embodiment 1
[0025] 1. Screening and identification of Parabacteroides distirii LCG-06
[0026] 1. Screening of Parabacteroides distirii LCG-06
[0027] Take intestinal feces from the intestines of pigs, mix 10g of feces and 100mL sterile water, filter to remove impurities, and make a feces dilution, and then continue to add sterile water to the dilution to prepare 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 diluent; then take 10 -2 、10 -3 、10 -4 、10 -5 Spread the dilution on soybean casein agar medium (TSA) in a medium containing 80% N 2 , 10% CO 2 and 10%H 2 Under an anaerobic environment, cultured at 37°C for 4 days, selected obvious colonies and purified them several times to obtain a single colony, which was named LCG-06, and then placed in soybean casein agar slant medium for refrigerated storage. The formula of the TSA medium is: tryptone 15.0g, soytone 5.0g, sodium chloride 5.0g, agar 15.0g, sterile water 1000mL, pH 7.3±0.2.
[0028] Such as figure 1 As shown, the biological cha...
Embodiment 2
[0048] In a farm in Shandong, 500 Boer goats were bred, of which 20 were randomly selected as the experimental group, 20 as the control group, and 40 goats were all 4-5 generation sheep. One month before the slaughter, the experimental group fed the goats with feed containing bile acid compound bacteria at noon every day, and mixed the bile acid compound bacteria into the feed at an amount of 30-50 mL per goat, and fed them continuously for 25 days , the sheep in the control group were fed the same amount of feed without adding bile acid compound bacteria agent. Concentrate feed and grass feed with the same mixing ratio were selected as feed, and all mutton sheep continued to be raised according to conventional breeding methods. Slaughtered after 25 days, the average slaughter weight of the mutton sheep in the control group was 56.44kg, and more yellow and white fat could be seen during slaughter; the average slaughter weight of the goats in the experimental group was 54.47 ca...
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